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Identification and purification of the specific antigen of Paracoccidioides brasiliensis responsible for immunoelectrophoretic band E.
Sabouraudia. 1977 Mar; 15(1):79-85.S

Abstract

A new purified antigen (E2) of Paracoccidioides brasiliensis mycelial growth phase was isolated by immunoadsorption from a crude metabolic soluble extract of the fungus. The antiserum prepared in a rabbit by inoculation of E2 antigen developed only one immunodiffusion line with the crude metabolic extract. Findings on immunological analysis showed that E2 antigen is the antigenic component of immunoelectrophoretic band E. The isolated antigens did not possess detectable alkaline phosphatase activity. It reacted in immunodiffusion tests with all the sera (14/14) from P. brasiliensis infected patients containing precipitating antibodies.

Authors

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Pub Type(s)

Journal Article

Language

eng

PubMed ID

404719

Citation

Yarzabal, L A., et al. "Identification and Purification of the Specific Antigen of Paracoccidioides Brasiliensis Responsible for Immunoelectrophoretic Band E." Sabouraudia, vol. 15, no. 1, 1977, pp. 79-85.
Yarzabal LA, Bout D, Naquira F, et al. Identification and purification of the specific antigen of Paracoccidioides brasiliensis responsible for immunoelectrophoretic band E. Sabouraudia. 1977;15(1):79-85.
Yarzabal, L. A., Bout, D., Naquira, F., Fruit, J., & Andrieu, S. (1977). Identification and purification of the specific antigen of Paracoccidioides brasiliensis responsible for immunoelectrophoretic band E. Sabouraudia, 15(1), 79-85.
Yarzabal LA, et al. Identification and Purification of the Specific Antigen of Paracoccidioides Brasiliensis Responsible for Immunoelectrophoretic Band E. Sabouraudia. 1977;15(1):79-85. PubMed PMID: 404719.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and purification of the specific antigen of Paracoccidioides brasiliensis responsible for immunoelectrophoretic band E. AU - Yarzabal,L A, AU - Bout,D, AU - Naquira,F, AU - Fruit,J, AU - Andrieu,S, PY - 1977/3/1/pubmed PY - 1977/3/1/medline PY - 1977/3/1/entrez SP - 79 EP - 85 JF - Sabouraudia JO - Sabouraudia VL - 15 IS - 1 N2 - A new purified antigen (E2) of Paracoccidioides brasiliensis mycelial growth phase was isolated by immunoadsorption from a crude metabolic soluble extract of the fungus. The antiserum prepared in a rabbit by inoculation of E2 antigen developed only one immunodiffusion line with the crude metabolic extract. Findings on immunological analysis showed that E2 antigen is the antigenic component of immunoelectrophoretic band E. The isolated antigens did not possess detectable alkaline phosphatase activity. It reacted in immunodiffusion tests with all the sera (14/14) from P. brasiliensis infected patients containing precipitating antibodies. SN - 0036-2174 UR - https://www.unboundmedicine.com/medline/citation/404719/Identification_and_purification_of_the_specific_antigen_of_Paracoccidioides_brasiliensis_responsible_for_immunoelectrophoretic_band_E_ DB - PRIME DP - Unbound Medicine ER -