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A qualitative and quantitative transmission electronmicroscopic study of the immediate pigment darkening reaction.
Photodermatol. 1985 Oct; 2(5):273-8.P

Abstract

Five healthy Caucasians, 3 having skin type II and 2 having skin type III, were UVA irradiated up to 14.5 J/cm2. Punch biopsies were taken before and immediately after irradiation and processed for transmission electron microscopy. Qualitative observations of the keratinocytes were obtained from the 5 subjects, while morphometry was performed on 1 subject with immediate pigment darkening reaction (IPD) (skin type III) and 1 without IPD (skin type II). The number of preformed melanosomes was 10-fold higher in the melanocytes and keratinocytes of the subject showing IPD as compared with the one who did not. No statistically significant difference, however, was observed in either skin type regarding the number of mature (stage IV) melanosomes of basal keratinocytes or melanocytes, size of melanosomes or redistribution of melanosomes in keratinocytes after UVA irradiation. No changes of the ultrastructure in the keratinocytes could be observed. It is concluded that the IPD consists of a photochemical reaction in preformed melanosomes and that the absence of IPD in skin type II could be due to the presence of too few preformed melanosomes.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

4070025

Citation

Beitner, H, and G Wennersten. "A Qualitative and Quantitative Transmission Electronmicroscopic Study of the Immediate Pigment Darkening Reaction." Photo-dermatology, vol. 2, no. 5, 1985, pp. 273-8.
Beitner H, Wennersten G. A qualitative and quantitative transmission electronmicroscopic study of the immediate pigment darkening reaction. Photodermatol. 1985;2(5):273-8.
Beitner, H., & Wennersten, G. (1985). A qualitative and quantitative transmission electronmicroscopic study of the immediate pigment darkening reaction. Photo-dermatology, 2(5), 273-8.
Beitner H, Wennersten G. A Qualitative and Quantitative Transmission Electronmicroscopic Study of the Immediate Pigment Darkening Reaction. Photodermatol. 1985;2(5):273-8. PubMed PMID: 4070025.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A qualitative and quantitative transmission electronmicroscopic study of the immediate pigment darkening reaction. AU - Beitner,H, AU - Wennersten,G, PY - 1985/10/1/pubmed PY - 1985/10/1/medline PY - 1985/10/1/entrez SP - 273 EP - 8 JF - Photo-dermatology JO - Photodermatol VL - 2 IS - 5 N2 - Five healthy Caucasians, 3 having skin type II and 2 having skin type III, were UVA irradiated up to 14.5 J/cm2. Punch biopsies were taken before and immediately after irradiation and processed for transmission electron microscopy. Qualitative observations of the keratinocytes were obtained from the 5 subjects, while morphometry was performed on 1 subject with immediate pigment darkening reaction (IPD) (skin type III) and 1 without IPD (skin type II). The number of preformed melanosomes was 10-fold higher in the melanocytes and keratinocytes of the subject showing IPD as compared with the one who did not. No statistically significant difference, however, was observed in either skin type regarding the number of mature (stage IV) melanosomes of basal keratinocytes or melanocytes, size of melanosomes or redistribution of melanosomes in keratinocytes after UVA irradiation. No changes of the ultrastructure in the keratinocytes could be observed. It is concluded that the IPD consists of a photochemical reaction in preformed melanosomes and that the absence of IPD in skin type II could be due to the presence of too few preformed melanosomes. SN - 0108-9684 UR - https://www.unboundmedicine.com/medline/citation/4070025/A_qualitative_and_quantitative_transmission_electronmicroscopic_study_of_the_immediate_pigment_darkening_reaction_ DB - PRIME DP - Unbound Medicine ER -