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65-70 kD protein identified by immunoblotting as the presumptive gastric microsomal autoantigen in pernicious anaemia.
Clin Exp Immunol. 1985 Dec; 62(3):732-7.CE

Abstract

Sera from 20 of 24 patients with pernicious anaemia reacted by immunoblotting with a 65-70 kD protein in canine and rodent gastric mucosal cells enriched for 80-90% parietal cells, and in microsomal preparations derived from these cells. All 20 reactive sera were positive for parietal cell microsomal antibody demonstrated by immunofluorescence. Eighteen parietal cell microsomal antibody-positive sera from patients with unconfirmed pernicious anaemia also reacted with the same 65-70 kD protein. Serum reactivity with the same 65-70 kD protein was not seen with canine and rodent liver cells or with microsomal preparations derived from these cells. Sera from 10 patients with chronic active hepatitis, 10 with scleroderma and 10 with rheumatoid arthritis and 22 healthy persons did not react with the 65-70 kD protein. These results suggest that the 65-70 kD protein is probably the parietal cell microsomal autoantigen. A second antigen of 85-90 kD mol. wt. present only in canine gastric mucosal preparations also correlates with the presence of parietal cell microsomal antibody. However, the contribution of this second antigen to parietal cell microsomal antibody reactivity remains uncertain.

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Authors

Dow CA
No affiliation info available
De Aizpurua HJ
No affiliation info available
Pedersen JS
No affiliation info available
Ungar B
No affiliation info available
Toh BH
No affiliation info available

MeSH

Anemia, PerniciousAnimalsAntigensAutoantigensDogsElectrophoresis, Polyacrylamide GelGastric MucosaGastritis, AtrophicHumansMiceMicrosomesMolecular WeightOrgan SpecificityParietal Cells, GastricProteinsRatsRats, Inbred Strains

Pub Type(s)

Journal Article

Language

eng

PubMed ID

4085153

Citation

Dow, C A., et al. "65-70 kD Protein Identified By Immunoblotting as the Presumptive Gastric Microsomal Autoantigen in Pernicious Anaemia." Clinical and Experimental Immunology, vol. 62, no. 3, 1985, pp. 732-7.
Dow CA, De Aizpurua HJ, Pedersen JS, et al. 65-70 kD protein identified by immunoblotting as the presumptive gastric microsomal autoantigen in pernicious anaemia. Clin Exp Immunol. 1985;62(3):732-7.
Dow, C. A., De Aizpurua, H. J., Pedersen, J. S., Ungar, B., & Toh, B. H. (1985). 65-70 kD protein identified by immunoblotting as the presumptive gastric microsomal autoantigen in pernicious anaemia. Clinical and Experimental Immunology, 62(3), 732-7.
Dow CA, et al. 65-70 kD Protein Identified By Immunoblotting as the Presumptive Gastric Microsomal Autoantigen in Pernicious Anaemia. Clin Exp Immunol. 1985;62(3):732-7. PubMed PMID: 4085153.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - 65-70 kD protein identified by immunoblotting as the presumptive gastric microsomal autoantigen in pernicious anaemia. AU - Dow,C A, AU - De Aizpurua,H J, AU - Pedersen,J S, AU - Ungar,B, AU - Toh,B H, PY - 1985/12/1/pubmed PY - 1985/12/1/medline PY - 1985/12/1/entrez SP - 732 EP - 7 JF - Clinical and experimental immunology JO - Clin Exp Immunol VL - 62 IS - 3 N2 - Sera from 20 of 24 patients with pernicious anaemia reacted by immunoblotting with a 65-70 kD protein in canine and rodent gastric mucosal cells enriched for 80-90% parietal cells, and in microsomal preparations derived from these cells. All 20 reactive sera were positive for parietal cell microsomal antibody demonstrated by immunofluorescence. Eighteen parietal cell microsomal antibody-positive sera from patients with unconfirmed pernicious anaemia also reacted with the same 65-70 kD protein. Serum reactivity with the same 65-70 kD protein was not seen with canine and rodent liver cells or with microsomal preparations derived from these cells. Sera from 10 patients with chronic active hepatitis, 10 with scleroderma and 10 with rheumatoid arthritis and 22 healthy persons did not react with the 65-70 kD protein. These results suggest that the 65-70 kD protein is probably the parietal cell microsomal autoantigen. A second antigen of 85-90 kD mol. wt. present only in canine gastric mucosal preparations also correlates with the presence of parietal cell microsomal antibody. However, the contribution of this second antigen to parietal cell microsomal antibody reactivity remains uncertain. SN - 0009-9104 UR - https://www.unboundmedicine.com/medline/citation/4085153/65_70_kD_protein_identified_by_immunoblotting_as_the_presumptive_gastric_microsomal_autoantigen_in_pernicious_anaemia_ DB - PRIME DP - Unbound Medicine ER -