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Aconitase and citric acid fermentation by Aspergillus niger.
Appl Environ Microbiol. 1985 Nov; 50(5):1336-8.AE

Abstract

In view of the often-cited theory that citric acid accumulation is caused by an inhibition of aconitase activity, the equilibrium of the reaction of aconitase was investigated by comparing in vivo steady-state concentrations of citrate and isocitrate in Aspergillus niger grown under various citric acid-producing conditions. With the equilibrium catalyzed by the A. niger enzyme in vitro, similar values were obtained. The validity of our in vivo measurements was verified by the addition of the aconitase inhibitor fluorocitrate, which appreciably elevated the citrate:isocitrate ratio. The results strongly argue against an inhibition of aconitase during citric acid fermentation.

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Authors

Kubicek CP
No affiliation info available
Röhr M
No affiliation info available

MeSH

Aconitate HydrataseAspergillus nigerCitratesCopperFermentationIronIsocitratesKinetics

Pub Type(s)

Journal Article

Language

eng

PubMed ID

4091562

Citation

Kubicek, C P., and M Röhr. "Aconitase and Citric Acid Fermentation By Aspergillus Niger." Applied and Environmental Microbiology, vol. 50, no. 5, 1985, pp. 1336-8.
Kubicek CP, Röhr M. Aconitase and citric acid fermentation by Aspergillus niger. Appl Environ Microbiol. 1985;50(5):1336-8.
Kubicek, C. P., & Röhr, M. (1985). Aconitase and citric acid fermentation by Aspergillus niger. Applied and Environmental Microbiology, 50(5), 1336-8.
Kubicek CP, Röhr M. Aconitase and Citric Acid Fermentation By Aspergillus Niger. Appl Environ Microbiol. 1985;50(5):1336-8. PubMed PMID: 4091562.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Aconitase and citric acid fermentation by Aspergillus niger. AU - Kubicek,C P, AU - Röhr,M, PY - 1985/11/1/pubmed PY - 1985/11/1/medline PY - 1985/11/1/entrez SP - 1336 EP - 8 JF - Applied and environmental microbiology JO - Appl Environ Microbiol VL - 50 IS - 5 N2 - In view of the often-cited theory that citric acid accumulation is caused by an inhibition of aconitase activity, the equilibrium of the reaction of aconitase was investigated by comparing in vivo steady-state concentrations of citrate and isocitrate in Aspergillus niger grown under various citric acid-producing conditions. With the equilibrium catalyzed by the A. niger enzyme in vitro, similar values were obtained. The validity of our in vivo measurements was verified by the addition of the aconitase inhibitor fluorocitrate, which appreciably elevated the citrate:isocitrate ratio. The results strongly argue against an inhibition of aconitase during citric acid fermentation. SN - 0099-2240 UR - https://www.unboundmedicine.com/medline/citation/4091562/Aconitase_and_citric_acid_fermentation_by_Aspergillus_niger_ DB - PRIME DP - Unbound Medicine ER -