TY - JOUR T1 - Isolation and characterization of a defective measles virus from brain biopsies of three patients in Iran with subacute sclerosing panencephalitis. AU - Mirchamsy,H, AU - Bahrami,S, AU - Shafyi,A, AU - Shahrabady,M S, AU - Kamaly,M, AU - Ahourai,P, AU - Razavi,J, AU - Nazari,P, AU - Derakhshan,I, AU - Lotfi,J, AU - Abassioun,K, PY - 1978/1/1/pubmed PY - 1978/1/1/medline PY - 1978/1/1/entrez SP - 106 EP - 18 JF - Intervirology JO - Intervirology VL - 9 IS - 2 N2 - Three cytopathic strains of subacute sclerosing panencephalitis (SSPE) virus were isolated from brain biopsies of three patients. These strains were isolated and maintained by cocultivation of infected brain cells with fresh Vero cells. The biological characteristics of two strains were studied. It was found that these strains remain cell-associated after repeated cocultivations with Vero cells and produce plaques under fluid medium or tragacanth overlay. The correlation with measles virus was demonstrated by the plaque reduction test as well as by the immunofluorescence test. Large numbers of nucleocapsids were observed in the cytoplasm of infected cells but none in nuclei. Intracerebral inoculation of monkeys, adult guinea pigs, newborn and adult hamsters or mice was followed by acute encephalitis and death. SN - 0300-5526 UR - https://www.unboundmedicine.com/medline/citation/412813/Isolation_and_characterization_of_a_defective_measles_virus_from_brain_biopsies_of_three_patients_in_Iran_with_subacute_sclerosing_panencephalitis_ L2 - https://www.karger.com?DOI=10.1159/000148928 DB - PRIME DP - Unbound Medicine ER -