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Identification of malaria parasites by fluorescence microscopy and acridine orange staining.
Bull World Health Organ. 1973 May; 48(5):591-6.BW

Abstract

The need for a technique that is more sensitive than the use of Romanowsky-stained thick blood films for detecting malaria parasites at low concentration in the blood is well recognized. One of the more promising methods appeared to be fluorochrome staining with acridine orange. However, reports on the efficacy of the technique were contradictory and it was not clear to what extent blood films taken under survey conditions would contain fluorescing artefacts that might confuse diagnosis. An investigation indicated that, provided reasonable care was taken, blood films made under survey conditions contained few confusing artefacts. However, it was found that, while acridine orange staining might have a slight advantage when large malaria parasites were present, it was inferior to routine Romanowsky staining for the detection of young trophozoites, the inferiority becoming more pronounced as the parasite concentration decreased.

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Authors

Shute GT
No affiliation info available
Sodeman TM
No affiliation info available

MeSH

AcridinesBacteriological TechniquesBloodHumansMalariaMicroscopy, FluorescencePhilippinesPlasmodium falciparumPlasmodium vivaxStaining and Labeling

Pub Type(s)

Journal Article

Language

eng

PubMed ID

4130021

Citation

Shute, G T., and T M. Sodeman. "Identification of Malaria Parasites By Fluorescence Microscopy and Acridine Orange Staining." Bulletin of the World Health Organization, vol. 48, no. 5, 1973, pp. 591-6.
Shute GT, Sodeman TM. Identification of malaria parasites by fluorescence microscopy and acridine orange staining. Bull World Health Organ. 1973;48(5):591-6.
Shute, G. T., & Sodeman, T. M. (1973). Identification of malaria parasites by fluorescence microscopy and acridine orange staining. Bulletin of the World Health Organization, 48(5), 591-6.
Shute GT, Sodeman TM. Identification of Malaria Parasites By Fluorescence Microscopy and Acridine Orange Staining. Bull World Health Organ. 1973;48(5):591-6. PubMed PMID: 4130021.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of malaria parasites by fluorescence microscopy and acridine orange staining. AU - Shute,G T, AU - Sodeman,T M, PY - 1973/5/1/pubmed PY - 1973/5/1/medline PY - 1973/5/1/entrez SP - 591 EP - 6 JF - Bulletin of the World Health Organization JO - Bull World Health Organ VL - 48 IS - 5 N2 - The need for a technique that is more sensitive than the use of Romanowsky-stained thick blood films for detecting malaria parasites at low concentration in the blood is well recognized. One of the more promising methods appeared to be fluorochrome staining with acridine orange. However, reports on the efficacy of the technique were contradictory and it was not clear to what extent blood films taken under survey conditions would contain fluorescing artefacts that might confuse diagnosis. An investigation indicated that, provided reasonable care was taken, blood films made under survey conditions contained few confusing artefacts. However, it was found that, while acridine orange staining might have a slight advantage when large malaria parasites were present, it was inferior to routine Romanowsky staining for the detection of young trophozoites, the inferiority becoming more pronounced as the parasite concentration decreased. SN - 0042-9686 UR - https://www.unboundmedicine.com/medline/citation/4130021/Identification_of_malaria_parasites_by_fluorescence_microscopy_and_acridine_orange_staining_ DB - PRIME DP - Unbound Medicine ER -