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Influence of glucagon, 6-N,2'-O-dibutyryladenosine 3':5'-cyclic monophosphate and triamcinolone on the arginine synthetase system in perinatal rat liver.
Biochem J. 1972 Jan; 126(1):89-98.BJ

Abstract

1. The administration of triamcinolone (19-190mug/animal) to postnatal rats increased the arginine synthetase system activity 1.2-2.5-fold above control values 24h after exposure to the hormone. Cortisol (hydrocortisone), however, increased the arginine synthetase system activity only when larger (190mug/animal) or repeated daily doses were given. Glucagon (100mug/animal) stimulated arginine synthetase system activity only after the second postnatal day. None of these agents increased the activity in 19.5-21.5-day foetuses after intrauterine administration. 2. The viability of foetal rat liver explants maintained in organ culture for up to 54h was validated both by ultramicroscopic examination and by incorporation of radioactive leucine and orotic acid. 3. In organ cultures of foetal rat liver explants (18.5 days to term), triamcinolone (20mug/ml of medium) evoked a 2.8-4.3-fold increase after 24h of incubation. This increase was completely inhibited by actinomycin D (25mug/ml) or cycloheximide (10mug/ml). Cortisol (5-50mug/ml) or glucagon (0.067-67mug/ml) also increased the arginine synthetase system activity above the respective control values, but there was no increase in activity with insulin (0.05-0.25i.u./ml). 4. Maximum concentrations of glucagon (67mug/ml), dibutyryl cyclic AMP (6-N,2'-O-dibutyryladenosine 3':5'-cyclic monophosphate) (0.1mm) and triamcinolone (20mug/ml) incubated for 24h with foetal rat liver explants each produced between a two-and three-fold increase in the activity of the arginine synthetase system. Combinations of maximum amounts of glucagon and the cyclic nucleotide did not produce a greater effect than either agent alone. However, the combination of dibutyryl cyclic AMP with triamcinolone appeared to produce somewhat less than additive effects. 5. The effects of the cyclic nucleotide and triamcinolone were evident after 12h of incubation and increased steadily throughout the 24h of observation. This time-course of increased enzyme activity is very much slower than that reported for the induction of other enzymes in explant cultures of foetal rat liver.

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Authors

Schwartz AL
No affiliation info available

MeSH

Adenine NucleotidesAnimalsCarbon IsotopesCitrullineCyclic AMPCycloheximideDactinomycinFemaleGestational AgeGlucagonHydrocortisoneLeucineLigasesLiverLyasesMicroscopy, ElectronOrgan Culture TechniquesOrotic AcidPregnancyRatsTriamcinolone

Pub Type(s)

Journal Article

Language

eng

PubMed ID

4342387

Citation

Schwartz, A L.. "Influence of Glucagon, 6-N,2'-O-dibutyryladenosine 3':5'-cyclic Monophosphate and Triamcinolone On the Arginine Synthetase System in Perinatal Rat Liver." The Biochemical Journal, vol. 126, no. 1, 1972, pp. 89-98.
Schwartz AL. Influence of glucagon, 6-N,2'-O-dibutyryladenosine 3':5'-cyclic monophosphate and triamcinolone on the arginine synthetase system in perinatal rat liver. Biochem J. 1972;126(1):89-98.
Schwartz, A. L. (1972). Influence of glucagon, 6-N,2'-O-dibutyryladenosine 3':5'-cyclic monophosphate and triamcinolone on the arginine synthetase system in perinatal rat liver. The Biochemical Journal, 126(1), 89-98.
Schwartz AL. Influence of Glucagon, 6-N,2'-O-dibutyryladenosine 3':5'-cyclic Monophosphate and Triamcinolone On the Arginine Synthetase System in Perinatal Rat Liver. Biochem J. 1972;126(1):89-98. PubMed PMID: 4342387.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Influence of glucagon, 6-N,2'-O-dibutyryladenosine 3':5'-cyclic monophosphate and triamcinolone on the arginine synthetase system in perinatal rat liver. A1 - Schwartz,A L, PY - 1972/1/1/pubmed PY - 1972/1/1/medline PY - 1972/1/1/entrez SP - 89 EP - 98 JF - The Biochemical journal JO - Biochem J VL - 126 IS - 1 N2 - 1. The administration of triamcinolone (19-190mug/animal) to postnatal rats increased the arginine synthetase system activity 1.2-2.5-fold above control values 24h after exposure to the hormone. Cortisol (hydrocortisone), however, increased the arginine synthetase system activity only when larger (190mug/animal) or repeated daily doses were given. Glucagon (100mug/animal) stimulated arginine synthetase system activity only after the second postnatal day. None of these agents increased the activity in 19.5-21.5-day foetuses after intrauterine administration. 2. The viability of foetal rat liver explants maintained in organ culture for up to 54h was validated both by ultramicroscopic examination and by incorporation of radioactive leucine and orotic acid. 3. In organ cultures of foetal rat liver explants (18.5 days to term), triamcinolone (20mug/ml of medium) evoked a 2.8-4.3-fold increase after 24h of incubation. This increase was completely inhibited by actinomycin D (25mug/ml) or cycloheximide (10mug/ml). Cortisol (5-50mug/ml) or glucagon (0.067-67mug/ml) also increased the arginine synthetase system activity above the respective control values, but there was no increase in activity with insulin (0.05-0.25i.u./ml). 4. Maximum concentrations of glucagon (67mug/ml), dibutyryl cyclic AMP (6-N,2'-O-dibutyryladenosine 3':5'-cyclic monophosphate) (0.1mm) and triamcinolone (20mug/ml) incubated for 24h with foetal rat liver explants each produced between a two-and three-fold increase in the activity of the arginine synthetase system. Combinations of maximum amounts of glucagon and the cyclic nucleotide did not produce a greater effect than either agent alone. However, the combination of dibutyryl cyclic AMP with triamcinolone appeared to produce somewhat less than additive effects. 5. The effects of the cyclic nucleotide and triamcinolone were evident after 12h of incubation and increased steadily throughout the 24h of observation. This time-course of increased enzyme activity is very much slower than that reported for the induction of other enzymes in explant cultures of foetal rat liver. SN - 0264-6021 UR - https://www.unboundmedicine.com/medline/citation/4342387/Influence_of_glucagon_6_N2'_O_dibutyryladenosine_3':5'_cyclic_monophosphate_and_triamcinolone_on_the_arginine_synthetase_system_in_perinatal_rat_liver_ DB - PRIME DP - Unbound Medicine ER -