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Trehalose metabolism by Bacillus popilliae.
J Bacteriol. 1974 Aug; 119(2):484-93.JB

Abstract

Trehalose was found to be utilized more readily than glucose for the growth of Bacillus popilliae NRRL B-2309MC. The pathway of degradation of trehalose was elucidated and found to differ from that reported for other organisms. Trehalase and trehalose phosphorylase activities could not be detected. Rather, trehalose was found to undergo phosphoenolpyruvate (PEP)-dependent phosphorylation, and the resulting trehalose 6-phosphate was cleaved by a phosphotrehalase to equimolar amounts of glucose and glucose 6-phosphate. The phosphotrehalase was purified 34-fold and shown to have a pH optimum of 6.5 to 7.0 and a K(m) for trehalose 6-phosphate of 1.8 mM. A mutant missing the phosphotrehalase failed to grow on trehalose but grew normally on other sugars. The mutant accumulated [(14)C]trehalose as [(14)C]trehalose 6-phosphate. Phosphorylation of trehalose by dialyzed extracts was at least 25 times faster with PEP than with adenosine 5'-triphosphate, and the phosphorylation activity was associated primarily with the particulate fraction. These data and the results of studies of [(14)C]trehalose uptake suggest that trehalose is transported into the cell as trehalose 6-phosphate by a PEP:sugar phosphotransferase system. Cell extracts of other strains of B. popilliae were also found to produce [(14)C]sugar phosphate from [(14)C]trehalose and to have phosphotrehalase activity.

Authors

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Pub Type(s)

Journal Article

Language

eng

PubMed ID

4369400

Citation

Bhumiratana, A, et al. "Trehalose Metabolism By Bacillus Popilliae." Journal of Bacteriology, vol. 119, no. 2, 1974, pp. 484-93.
Bhumiratana A, Anderson RL, Costilow RN. Trehalose metabolism by Bacillus popilliae. J Bacteriol. 1974;119(2):484-93.
Bhumiratana, A., Anderson, R. L., & Costilow, R. N. (1974). Trehalose metabolism by Bacillus popilliae. Journal of Bacteriology, 119(2), 484-93.
Bhumiratana A, Anderson RL, Costilow RN. Trehalose Metabolism By Bacillus Popilliae. J Bacteriol. 1974;119(2):484-93. PubMed PMID: 4369400.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Trehalose metabolism by Bacillus popilliae. AU - Bhumiratana,A, AU - Anderson,R L, AU - Costilow,R N, PY - 1974/8/1/pubmed PY - 1974/8/1/medline PY - 1974/8/1/entrez SP - 484 EP - 93 JF - Journal of bacteriology JO - J Bacteriol VL - 119 IS - 2 N2 - Trehalose was found to be utilized more readily than glucose for the growth of Bacillus popilliae NRRL B-2309MC. The pathway of degradation of trehalose was elucidated and found to differ from that reported for other organisms. Trehalase and trehalose phosphorylase activities could not be detected. Rather, trehalose was found to undergo phosphoenolpyruvate (PEP)-dependent phosphorylation, and the resulting trehalose 6-phosphate was cleaved by a phosphotrehalase to equimolar amounts of glucose and glucose 6-phosphate. The phosphotrehalase was purified 34-fold and shown to have a pH optimum of 6.5 to 7.0 and a K(m) for trehalose 6-phosphate of 1.8 mM. A mutant missing the phosphotrehalase failed to grow on trehalose but grew normally on other sugars. The mutant accumulated [(14)C]trehalose as [(14)C]trehalose 6-phosphate. Phosphorylation of trehalose by dialyzed extracts was at least 25 times faster with PEP than with adenosine 5'-triphosphate, and the phosphorylation activity was associated primarily with the particulate fraction. These data and the results of studies of [(14)C]trehalose uptake suggest that trehalose is transported into the cell as trehalose 6-phosphate by a PEP:sugar phosphotransferase system. Cell extracts of other strains of B. popilliae were also found to produce [(14)C]sugar phosphate from [(14)C]trehalose and to have phosphotrehalase activity. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/4369400/Trehalose_metabolism_by_Bacillus_popilliae_ L2 - https://journals.asm.org/doi/10.1128/jb.119.2.484-493.1974?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -