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Adenosine triphosphate-linked control of Pseudomonas aeruginosa glucose-6-phosphate dehydrogenase.
J Bacteriol. 1967 Apr; 93(4):1337-45.JB

Abstract

Extracts of Pseudomonas aeruginosa (ATCC 7700) cells grown on glucose, gluconate, or glycerol had enzyme activities related to the Entner-Doudoroff pathway. These activities were present in no more than trace amounts when the bacteria were grown on succinate. Fructose-1,6-diphosphate aldolase could not be detected in extracts of the bacteria grown on any of the above carbon sources. Therefore, it appears that P. aeruginosa degrades glucose via an inducible Entner-Doudoroff pathway. The apparent absence of fructose-1,6-diphosphate aldolase in cells growing on succinate suggests that the bacteria can form hexose and pentose phosphates from succinate by an alternate route. d-Glucose-6-phosphate dehydrogenase, a branch-point enzyme of the Entner-Doudoroff pathway, was purified 50-fold from glucose-grown cells. Its molecular weight, estimated by sucrose density gradient centrifugation, was found to be approximately 190,000. The enzyme was strongly inhibited by adenosine triphosphate, guanosine triphosphate, and deoxyguanosine triphosphate, which decreased the apparent binding of glucose-6-phosphate to the enzyme. It is suggested that adenine nucleotide-linked control of glucose-6-phosphate dehydrogenase may regulate the overall catabolism of hexose phosphates and prevent their wasteful degradation under certain conditions requiring gluconeogenesis.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

4382249

Citation

Lessie, T, and F C. Neidhardt. "Adenosine Triphosphate-linked Control of Pseudomonas Aeruginosa Glucose-6-phosphate Dehydrogenase." Journal of Bacteriology, vol. 93, no. 4, 1967, pp. 1337-45.
Lessie T, Neidhardt FC. Adenosine triphosphate-linked control of Pseudomonas aeruginosa glucose-6-phosphate dehydrogenase. J Bacteriol. 1967;93(4):1337-45.
Lessie, T., & Neidhardt, F. C. (1967). Adenosine triphosphate-linked control of Pseudomonas aeruginosa glucose-6-phosphate dehydrogenase. Journal of Bacteriology, 93(4), 1337-45.
Lessie T, Neidhardt FC. Adenosine Triphosphate-linked Control of Pseudomonas Aeruginosa Glucose-6-phosphate Dehydrogenase. J Bacteriol. 1967;93(4):1337-45. PubMed PMID: 4382249.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Adenosine triphosphate-linked control of Pseudomonas aeruginosa glucose-6-phosphate dehydrogenase. AU - Lessie,T, AU - Neidhardt,F C, PY - 1967/4/1/pubmed PY - 1967/4/1/medline PY - 1967/4/1/entrez SP - 1337 EP - 45 JF - Journal of bacteriology JO - J. Bacteriol. VL - 93 IS - 4 N2 - Extracts of Pseudomonas aeruginosa (ATCC 7700) cells grown on glucose, gluconate, or glycerol had enzyme activities related to the Entner-Doudoroff pathway. These activities were present in no more than trace amounts when the bacteria were grown on succinate. Fructose-1,6-diphosphate aldolase could not be detected in extracts of the bacteria grown on any of the above carbon sources. Therefore, it appears that P. aeruginosa degrades glucose via an inducible Entner-Doudoroff pathway. The apparent absence of fructose-1,6-diphosphate aldolase in cells growing on succinate suggests that the bacteria can form hexose and pentose phosphates from succinate by an alternate route. d-Glucose-6-phosphate dehydrogenase, a branch-point enzyme of the Entner-Doudoroff pathway, was purified 50-fold from glucose-grown cells. Its molecular weight, estimated by sucrose density gradient centrifugation, was found to be approximately 190,000. The enzyme was strongly inhibited by adenosine triphosphate, guanosine triphosphate, and deoxyguanosine triphosphate, which decreased the apparent binding of glucose-6-phosphate to the enzyme. It is suggested that adenine nucleotide-linked control of glucose-6-phosphate dehydrogenase may regulate the overall catabolism of hexose phosphates and prevent their wasteful degradation under certain conditions requiring gluconeogenesis. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/4382249/Adenosine_triphosphate_linked_control_of_Pseudomonas_aeruginosa_glucose_6_phosphate_dehydrogenase_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=4382249 DB - PRIME DP - Unbound Medicine ER -