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Biochemistry of Coxiella burnetii: 6-phosphogluconic acid dehydrogenase.
J Bacteriol. 1970 Apr; 102(1):1-5.JB

Abstract

Purified preparations of the rickettsial agent, Coxiella burnetii, have been examined for their ability to decarboxylate 6-phosphogluconate. The enzyme 6-phosphogluconic acid dehydrogenase [6-phospho-d-gluconate: NADP (nicotinamide adenine dinucleotide phosphate) oxidoreductase (decarboxylating), EC 1.1.1.44] was detected in extracts, but not in whole-cell preparations of C. burnetii. Both extracts and whole cells were shown to be free from contaminating host enzyme activity. Partial characterization of the enzyme has shown that it is substrate-dependent, specific for NADP, and requires magnesium for activity. The pH optimum of the rickettsial enzyme is 8.0.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

4392386

Citation

McDonald, T L., and L Mallavia. "Biochemistry of Coxiella Burnetii: 6-phosphogluconic Acid Dehydrogenase." Journal of Bacteriology, vol. 102, no. 1, 1970, pp. 1-5.
McDonald TL, Mallavia L. Biochemistry of Coxiella burnetii: 6-phosphogluconic acid dehydrogenase. J Bacteriol. 1970;102(1):1-5.
McDonald, T. L., & Mallavia, L. (1970). Biochemistry of Coxiella burnetii: 6-phosphogluconic acid dehydrogenase. Journal of Bacteriology, 102(1), 1-5.
McDonald TL, Mallavia L. Biochemistry of Coxiella Burnetii: 6-phosphogluconic Acid Dehydrogenase. J Bacteriol. 1970;102(1):1-5. PubMed PMID: 4392386.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biochemistry of Coxiella burnetii: 6-phosphogluconic acid dehydrogenase. AU - McDonald,T L, AU - Mallavia,L, PY - 1970/4/1/pubmed PY - 1970/4/1/medline PY - 1970/4/1/entrez SP - 1 EP - 5 JF - Journal of bacteriology JO - J. Bacteriol. VL - 102 IS - 1 N2 - Purified preparations of the rickettsial agent, Coxiella burnetii, have been examined for their ability to decarboxylate 6-phosphogluconate. The enzyme 6-phosphogluconic acid dehydrogenase [6-phospho-d-gluconate: NADP (nicotinamide adenine dinucleotide phosphate) oxidoreductase (decarboxylating), EC 1.1.1.44] was detected in extracts, but not in whole-cell preparations of C. burnetii. Both extracts and whole cells were shown to be free from contaminating host enzyme activity. Partial characterization of the enzyme has shown that it is substrate-dependent, specific for NADP, and requires magnesium for activity. The pH optimum of the rickettsial enzyme is 8.0. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/4392386/Biochemistry_of_Coxiella_burnetii:_6_phosphogluconic_acid_dehydrogenase_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=4392386 DB - PRIME DP - Unbound Medicine ER -