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Heterotrophic metabolism of the chemolithotroph Thiobacillus ferrooxidans.
J Bacteriol. 1971 Oct; 108(1):334-42.JB

Abstract

Glucose-6-phosphate dehydrogenase and the enzymes of the Entner-Doudoroff pathway, 6-phosphogluconate dehydrase and 2-keto-3-deoxy-6-phosphogluconate aldolase (assayed together), are induced during heterotrophic growth of Thiobacillus ferrooxidans on an iron-glucose-supplemented medium or on glucose alone. By contrast, autotrophic cells (iron-grown) contain low levels of these enzymes. Fructose 1, 6-diphosphate aldolase, an enzyme of the Embden-Meyerhof pathway, is present at low levels irrespective of the growth medium, suggesting that this enzyme is not involved in energy-yielding reactions but merely provides intermediates for biosynthesis. The Entner-Doudoroff and pentose-phosphate pathways are the principle means through which glucose is dissimilated and is presumed to be concerned with energy production. Isotopic studies showed that a high rate of CO(2) formation from specifically labeled glucose came from carbon atoms 1 and 4. An unexpectedly high rate of evolution of CO(2) also came from carbon 6, suggesting that the triose phosphate formed during glucose breakdown and specifically as a result of 2-keto-3-deoxy-6-phosphogluconate aldolase activity, was metabolized via some unorthodox metabolic route. Cells grown in the iron-supplemented and glucose-salts media have a complete tricarboxylic acid cycle, whereas autotrophically grown T. ferrooxidans lacked both alpha-ketoglutarate dehydrogenase and reduced nicotinamide adenine dinucleotide oxidase. Two isocitrate dehydrogenases [nicotinamide adenine dinucleotide (NAD) and NAD phosphate (NADP) specific] were present. NAD-linked enzyme was constitutive, whereas the NADP-linked enzyme was induced upon adaptation of autotrophic cells to heterotrophic growth.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

4399339

Citation

Tabita, R, and D G. Lundgren. "Heterotrophic Metabolism of the Chemolithotroph Thiobacillus Ferrooxidans." Journal of Bacteriology, vol. 108, no. 1, 1971, pp. 334-42.
Tabita R, Lundgren DG. Heterotrophic metabolism of the chemolithotroph Thiobacillus ferrooxidans. J Bacteriol. 1971;108(1):334-42.
Tabita, R., & Lundgren, D. G. (1971). Heterotrophic metabolism of the chemolithotroph Thiobacillus ferrooxidans. Journal of Bacteriology, 108(1), 334-42.
Tabita R, Lundgren DG. Heterotrophic Metabolism of the Chemolithotroph Thiobacillus Ferrooxidans. J Bacteriol. 1971;108(1):334-42. PubMed PMID: 4399339.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Heterotrophic metabolism of the chemolithotroph Thiobacillus ferrooxidans. AU - Tabita,R, AU - Lundgren,D G, PY - 1971/10/1/pubmed PY - 1971/10/1/medline PY - 1971/10/1/entrez SP - 334 EP - 42 JF - Journal of bacteriology JO - J. Bacteriol. VL - 108 IS - 1 N2 - Glucose-6-phosphate dehydrogenase and the enzymes of the Entner-Doudoroff pathway, 6-phosphogluconate dehydrase and 2-keto-3-deoxy-6-phosphogluconate aldolase (assayed together), are induced during heterotrophic growth of Thiobacillus ferrooxidans on an iron-glucose-supplemented medium or on glucose alone. By contrast, autotrophic cells (iron-grown) contain low levels of these enzymes. Fructose 1, 6-diphosphate aldolase, an enzyme of the Embden-Meyerhof pathway, is present at low levels irrespective of the growth medium, suggesting that this enzyme is not involved in energy-yielding reactions but merely provides intermediates for biosynthesis. The Entner-Doudoroff and pentose-phosphate pathways are the principle means through which glucose is dissimilated and is presumed to be concerned with energy production. Isotopic studies showed that a high rate of CO(2) formation from specifically labeled glucose came from carbon atoms 1 and 4. An unexpectedly high rate of evolution of CO(2) also came from carbon 6, suggesting that the triose phosphate formed during glucose breakdown and specifically as a result of 2-keto-3-deoxy-6-phosphogluconate aldolase activity, was metabolized via some unorthodox metabolic route. Cells grown in the iron-supplemented and glucose-salts media have a complete tricarboxylic acid cycle, whereas autotrophically grown T. ferrooxidans lacked both alpha-ketoglutarate dehydrogenase and reduced nicotinamide adenine dinucleotide oxidase. Two isocitrate dehydrogenases [nicotinamide adenine dinucleotide (NAD) and NAD phosphate (NADP) specific] were present. NAD-linked enzyme was constitutive, whereas the NADP-linked enzyme was induced upon adaptation of autotrophic cells to heterotrophic growth. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/4399339/Heterotrophic_metabolism_of_the_chemolithotroph_Thiobacillus_ferrooxidans_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=4399339 DB - PRIME DP - Unbound Medicine ER -