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Induction and regulation of a nicotinamide adenine dinucleotide-specific 6-phosphogluconate dehydrogenase in Streptococcus faecalis.
J Bacteriol. 1972 Jan; 109(1):106-15.JB

Abstract

Streptococcus faecalis grown with glucose as the primary energy source contains a single, nicotinamide adenine dinucleotide phosphate (NADP)-specific 6-phosphogluconate dehydrogenase. Extracts of gluconate-adapted cells, however, exhibited 6-phosphogluconate dehydrogenase activity with either NADP or nicotinamide adenine dinucleotide (NAD). This was shown to be due to the presence of separate enzymes in gluconate-adapted cells. Although both enzymes catalyzed the oxidative decarboxylation of 6-phosphogluconate, they differed from one another with respect to their coenzyme specificity, molecular weight, pH optimum, K(m) values for substrate and coenzyme, and electrophoretic mobility in starch gels. The two enzymes also differed in their response to certain effector ligands. The NADP-linked enzyme was specifically inhibited by fructose-1,6-diphosphate, but was insensitive to adenosine triphosphate (ATP) and certain other nucleotides. The NAD-specific enzyme, in contrast, was insensitive to fructose-1,6-diphosphate, but was inhibited by ATP. The available data suggest that the NAD enzyme is involved primarily in the catabolism of gluconate, whereas the NADP enzyme appears to function in the production of reducing equivalents (NADPH) for use in various reductive biosynthetic reactions.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

4400413

Citation

Brown, A T., and C L. Wittenberger. "Induction and Regulation of a Nicotinamide Adenine Dinucleotide-specific 6-phosphogluconate Dehydrogenase in Streptococcus Faecalis." Journal of Bacteriology, vol. 109, no. 1, 1972, pp. 106-15.
Brown AT, Wittenberger CL. Induction and regulation of a nicotinamide adenine dinucleotide-specific 6-phosphogluconate dehydrogenase in Streptococcus faecalis. J Bacteriol. 1972;109(1):106-15.
Brown, A. T., & Wittenberger, C. L. (1972). Induction and regulation of a nicotinamide adenine dinucleotide-specific 6-phosphogluconate dehydrogenase in Streptococcus faecalis. Journal of Bacteriology, 109(1), 106-15.
Brown AT, Wittenberger CL. Induction and Regulation of a Nicotinamide Adenine Dinucleotide-specific 6-phosphogluconate Dehydrogenase in Streptococcus Faecalis. J Bacteriol. 1972;109(1):106-15. PubMed PMID: 4400413.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Induction and regulation of a nicotinamide adenine dinucleotide-specific 6-phosphogluconate dehydrogenase in Streptococcus faecalis. AU - Brown,A T, AU - Wittenberger,C L, PY - 1972/1/1/pubmed PY - 1972/1/1/medline PY - 1972/1/1/entrez SP - 106 EP - 15 JF - Journal of bacteriology JO - J. Bacteriol. VL - 109 IS - 1 N2 - Streptococcus faecalis grown with glucose as the primary energy source contains a single, nicotinamide adenine dinucleotide phosphate (NADP)-specific 6-phosphogluconate dehydrogenase. Extracts of gluconate-adapted cells, however, exhibited 6-phosphogluconate dehydrogenase activity with either NADP or nicotinamide adenine dinucleotide (NAD). This was shown to be due to the presence of separate enzymes in gluconate-adapted cells. Although both enzymes catalyzed the oxidative decarboxylation of 6-phosphogluconate, they differed from one another with respect to their coenzyme specificity, molecular weight, pH optimum, K(m) values for substrate and coenzyme, and electrophoretic mobility in starch gels. The two enzymes also differed in their response to certain effector ligands. The NADP-linked enzyme was specifically inhibited by fructose-1,6-diphosphate, but was insensitive to adenosine triphosphate (ATP) and certain other nucleotides. The NAD-specific enzyme, in contrast, was insensitive to fructose-1,6-diphosphate, but was inhibited by ATP. The available data suggest that the NAD enzyme is involved primarily in the catabolism of gluconate, whereas the NADP enzyme appears to function in the production of reducing equivalents (NADPH) for use in various reductive biosynthetic reactions. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/4400413/Induction_and_regulation_of_a_nicotinamide_adenine_dinucleotide_specific_6_phosphogluconate_dehydrogenase_in_Streptococcus_faecalis_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=4400413 DB - PRIME DP - Unbound Medicine ER -