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Spectrophotometric measurement of carboxyhemoglobin and methemoglobin in blood.
Clin Chem. 1979 Aug; 25(8):1388-93.CC

Abstract

This paper describes separate spectrophotometric procedures for rapidly measuring carboxyhemoglobin (COHb) and methemoglobin (MetHb) in blood. Absorbance measurements are made in the Soret region at a blood dilution near 1000-fold. For COHb estimation, the diluent contains sodium hydrosulfite, providing the two-component system COHb--Hb for absorbance measurements at 420 and 432 nm. The NA2S2O4 effectively prevents dissociation of COHb by oxygen. For MetHb estimation, the diluent contains KCN and carbon monoxide, providing the two-component system COHb--CNMetHb. Absorbance measurements are made at 420 nm, before and after addiely analyzed for the total of all MetHb forms present. Each procedure requires about 3 muL of blood and can be applied to human or animal blood. Results obtained by the present methods are in satisfactory agreement with currently accepted procedures, which require much larger samples or more elaborate equipment.

Authors

Rodkey FL
No affiliation info available
Hill TA
No affiliation info available
Pitts LL
No affiliation info available
Robertson RF
No affiliation info available

MeSH

CarboxyhemoglobinChromatography, GasHemoglobinsHumansIndicators and ReagentsMethemoglobinSpectrophotometry

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

455674

Citation

Rodkey, F L., et al. "Spectrophotometric Measurement of Carboxyhemoglobin and Methemoglobin in Blood." Clinical Chemistry, vol. 25, no. 8, 1979, pp. 1388-93.
Rodkey FL, Hill TA, Pitts LL, et al. Spectrophotometric measurement of carboxyhemoglobin and methemoglobin in blood. Clin Chem. 1979;25(8):1388-93.
Rodkey, F. L., Hill, T. A., Pitts, L. L., & Robertson, R. F. (1979). Spectrophotometric measurement of carboxyhemoglobin and methemoglobin in blood. Clinical Chemistry, 25(8), 1388-93.
Rodkey FL, et al. Spectrophotometric Measurement of Carboxyhemoglobin and Methemoglobin in Blood. Clin Chem. 1979;25(8):1388-93. PubMed PMID: 455674.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Spectrophotometric measurement of carboxyhemoglobin and methemoglobin in blood. AU - Rodkey,F L, AU - Hill,T A, AU - Pitts,L L, AU - Robertson,R F, PY - 1979/8/1/pubmed PY - 1979/8/1/medline PY - 1979/8/1/entrez SP - 1388 EP - 93 JF - Clinical chemistry JO - Clin Chem VL - 25 IS - 8 N2 - This paper describes separate spectrophotometric procedures for rapidly measuring carboxyhemoglobin (COHb) and methemoglobin (MetHb) in blood. Absorbance measurements are made in the Soret region at a blood dilution near 1000-fold. For COHb estimation, the diluent contains sodium hydrosulfite, providing the two-component system COHb--Hb for absorbance measurements at 420 and 432 nm. The NA2S2O4 effectively prevents dissociation of COHb by oxygen. For MetHb estimation, the diluent contains KCN and carbon monoxide, providing the two-component system COHb--CNMetHb. Absorbance measurements are made at 420 nm, before and after addiely analyzed for the total of all MetHb forms present. Each procedure requires about 3 muL of blood and can be applied to human or animal blood. Results obtained by the present methods are in satisfactory agreement with currently accepted procedures, which require much larger samples or more elaborate equipment. SN - 0009-9147 UR - https://www.unboundmedicine.com/medline/citation/455674/Spectrophotometric_measurement_of_carboxyhemoglobin_and_methemoglobin_in_blood_ L2 - https://www.lens.org/lens/search/patent/list?q=citation_id:455674 DB - PRIME DP - Unbound Medicine ER -