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Comparison of radioimmunoassay and gas chromatographic mass spectrometric assay for d-amphetamine.
Biomed Mass Spectrom. 1979 May; 6(5):187-90.BM

Abstract

Quantification of low levels of psychotropic drugs (10(-7) to 10(-9) g ml-1) in small volumes of plasma requires sensitive and accurate methods. Validation of these methods is best achieved by comparing results obtained using several techniques. In this study, amphetamine levels in plasma were measured using gas chromatography mass spectrometry and radioimmunoassay. Correlation of the results obtained by the two methods was found to be positive and high (R = 0.9822). The average coefficient of variation between assays for gas chromatography mass spectrometry was 5.8% and for radioimmunoassay was 12.3%, while the average coefficient of variation within assays for gas chromatography mass spectrometry was 4.9% and for radioimmunoassay 6.9%. Although gas chromatography mass spectrometry was 1.9 times more sensitive than radioimmunoassay, for most purposes, the convenience of the radioimmunoassay method outweights the technical superiority of gas chromatography mass spectrometry.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

476280

Citation

Powers, K H., and M H. Ebert. "Comparison of Radioimmunoassay and Gas Chromatographic Mass Spectrometric Assay for D-amphetamine." Biomedical Mass Spectrometry, vol. 6, no. 5, 1979, pp. 187-90.
Powers KH, Ebert MH. Comparison of radioimmunoassay and gas chromatographic mass spectrometric assay for d-amphetamine. Biomed Mass Spectrom. 1979;6(5):187-90.
Powers, K. H., & Ebert, M. H. (1979). Comparison of radioimmunoassay and gas chromatographic mass spectrometric assay for d-amphetamine. Biomedical Mass Spectrometry, 6(5), 187-90.
Powers KH, Ebert MH. Comparison of Radioimmunoassay and Gas Chromatographic Mass Spectrometric Assay for D-amphetamine. Biomed Mass Spectrom. 1979;6(5):187-90. PubMed PMID: 476280.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of radioimmunoassay and gas chromatographic mass spectrometric assay for d-amphetamine. AU - Powers,K H, AU - Ebert,M H, PY - 1979/5/1/pubmed PY - 1979/5/1/medline PY - 1979/5/1/entrez SP - 187 EP - 90 JF - Biomedical mass spectrometry JO - Biomed Mass Spectrom VL - 6 IS - 5 N2 - Quantification of low levels of psychotropic drugs (10(-7) to 10(-9) g ml-1) in small volumes of plasma requires sensitive and accurate methods. Validation of these methods is best achieved by comparing results obtained using several techniques. In this study, amphetamine levels in plasma were measured using gas chromatography mass spectrometry and radioimmunoassay. Correlation of the results obtained by the two methods was found to be positive and high (R = 0.9822). The average coefficient of variation between assays for gas chromatography mass spectrometry was 5.8% and for radioimmunoassay was 12.3%, while the average coefficient of variation within assays for gas chromatography mass spectrometry was 4.9% and for radioimmunoassay 6.9%. Although gas chromatography mass spectrometry was 1.9 times more sensitive than radioimmunoassay, for most purposes, the convenience of the radioimmunoassay method outweights the technical superiority of gas chromatography mass spectrometry. SN - 0306-042X UR - https://www.unboundmedicine.com/medline/citation/476280/Comparison_of_radioimmunoassay_and_gas_chromatographic_mass_spectrometric_assay_for_d_amphetamine_ DB - PRIME DP - Unbound Medicine ER -
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