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Experimental infection of chimpanzees with antihemophilic (factor VIII) materials: recovery of virus-like particles associated with non-A, non-B hepatitis.

Abstract

Non-A, non-B viral hepatitis was transmitted to four colony-born chimpanzees by infusion of three lots of antihemophilic factor (factor VIII) implicated in the transmission of non-A, non-B hepatitis to two human recipients. All four inoculated animals showed histopathological evidence of viral hepatitis, and all demonstrated significant ALT elevations between seven and one-half weeks after inoculation. Acute-phase plasma from one of the infected chimpanzees (no. 771) was shown to induce non-A, non-B hepatitis in two other chimpanzees approximately three weeks after their inoculation. In addition, an acute-phase open liver wedge biopsy obtained from animal no. 771 was processed and examined by immune electron microscopy (IEM) for virus-like particles with convalescent serum from a serologically confirmed case of non-A, non-B hepatitis. Twenty-five to 30 nm (mean = 27 nm) diameter virus-like particles that were either "full" or "empty" were identified in this liver preparation by IEM. Two additional chimpanzees inoculated with a cesium chloride gradient fraction of an isopycnically banded liver homogenate (animal no. 771) also developed elevated ALT activity two to two and one-half weeks later. Our findings have experimentally verified that commercially produced factor VIII materials can induce non-A, non-B hepatitis in champanzees and that the disease can be subpassaged in these animals by inoculation of either acute-phase plasma or liver. These results also provide evidence for the association of 27 nm-diameter virus-like particles with non-A, non-B viral hepatitis.

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  • Authors

    , , , , , , , , , ,

    Source

    Journal of medical virology 3:4 1979 pg 253-69

    MeSH

    Adult
    Alanine Transaminase
    Animals
    Factor VIII
    Female
    Hepatitis Viruses
    Hepatitis, Viral, Human
    Humans
    Liver
    Male
    Pan troglodytes

    Pub Type(s)

    Case Reports
    Journal Article

    Language

    eng

    PubMed ID

    479861

    Citation

    Bradley, D W., et al. "Experimental Infection of Chimpanzees With Antihemophilic (factor VIII) Materials: Recovery of Virus-like Particles Associated With non-A, non-B Hepatitis." Journal of Medical Virology, vol. 3, no. 4, 1979, pp. 253-69.
    Bradley DW, Cook EH, Maynard JE, et al. Experimental infection of chimpanzees with antihemophilic (factor VIII) materials: recovery of virus-like particles associated with non-A, non-B hepatitis. J Med Virol. 1979;3(4):253-69.
    Bradley, D. W., Cook, E. H., Maynard, J. E., McCaustland, K. A., Ebert, J. W., Dolana, G. H., ... Murphy, B. L. (1979). Experimental infection of chimpanzees with antihemophilic (factor VIII) materials: recovery of virus-like particles associated with non-A, non-B hepatitis. Journal of Medical Virology, 3(4), pp. 253-69.
    Bradley DW, et al. Experimental Infection of Chimpanzees With Antihemophilic (factor VIII) Materials: Recovery of Virus-like Particles Associated With non-A, non-B Hepatitis. J Med Virol. 1979;3(4):253-69. PubMed PMID: 479861.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Experimental infection of chimpanzees with antihemophilic (factor VIII) materials: recovery of virus-like particles associated with non-A, non-B hepatitis. AU - Bradley,D W, AU - Cook,E H, AU - Maynard,J E, AU - McCaustland,K A, AU - Ebert,J W, AU - Dolana,G H, AU - Petzel,R A, AU - Kantor,R J, AU - Heilbrunn,A, AU - Fields,H A, AU - Murphy,B L, PY - 1979/1/1/pubmed PY - 1979/1/1/medline PY - 1979/1/1/entrez SP - 253 EP - 69 JF - Journal of medical virology JO - J. Med. Virol. VL - 3 IS - 4 N2 - Non-A, non-B viral hepatitis was transmitted to four colony-born chimpanzees by infusion of three lots of antihemophilic factor (factor VIII) implicated in the transmission of non-A, non-B hepatitis to two human recipients. All four inoculated animals showed histopathological evidence of viral hepatitis, and all demonstrated significant ALT elevations between seven and one-half weeks after inoculation. Acute-phase plasma from one of the infected chimpanzees (no. 771) was shown to induce non-A, non-B hepatitis in two other chimpanzees approximately three weeks after their inoculation. In addition, an acute-phase open liver wedge biopsy obtained from animal no. 771 was processed and examined by immune electron microscopy (IEM) for virus-like particles with convalescent serum from a serologically confirmed case of non-A, non-B hepatitis. Twenty-five to 30 nm (mean = 27 nm) diameter virus-like particles that were either "full" or "empty" were identified in this liver preparation by IEM. Two additional chimpanzees inoculated with a cesium chloride gradient fraction of an isopycnically banded liver homogenate (animal no. 771) also developed elevated ALT activity two to two and one-half weeks later. Our findings have experimentally verified that commercially produced factor VIII materials can induce non-A, non-B hepatitis in champanzees and that the disease can be subpassaged in these animals by inoculation of either acute-phase plasma or liver. These results also provide evidence for the association of 27 nm-diameter virus-like particles with non-A, non-B viral hepatitis. SN - 0146-6615 UR - https://www.unboundmedicine.com/medline/citation/479861/Experimental_infection_of_chimpanzees_with_antihemophilic__factor_VIII__materials:_recovery_of_virus_like_particles_associated_with_non_A_non_B_hepatitis_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0146-6615&date=1979&volume=3&issue=4&spage=253 DB - PRIME DP - Unbound Medicine ER -