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Mechanism by which hydnocarpic acid inhibits mycobacterial multiplication.
Antimicrob Agents Chemother 1973; 3(3):373-9AA

Abstract

Recent work in this laboratory has shown that hydnocarpic acid (HA), a principal constituent of chaulmoogra oil, inhibits multiplication in vitro of a number of mycobacterial species. This activity of HA was not shared by several straight-chain fatty acids and by dihydrochaulmoogric acid. A study of the interaction of HA with biotin has been undertaken, based on a structural analogy between biotin and the cyclopentenyl fatty acid. The multiplication of a strain of Mycobacterium intracellulare susceptible to 2 mug of HA/ml was measured turbidimetrically in Dubos medium, in the presence and absence of biotin and several other compounds. Biotin and, to a lesser extent, adenine plus guanine, palmitic acid, and linoleic acid antagonized growth inhibition by HA. Desthiobiotin, thioctic acid, and succinic acid did not block inhibition of bacterial multiplication by HA. HA may act by blocking the coenzymatic activity of biotin, or it may inhibit microbial biotin synthesis. Resumption of multiplication of M. intracellulare after a period of inhibition by HA in broth culture was found to be accompanied by reduction of the effective concentration of the drug; this could have resulted from metabolism of HA or production of an antagonist to HA by the organisms. Also, those organisms that multiplied in the presence of HA were found to represent HA-resistant mutants of M. intracellulare.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

4799554

Citation

Jacobsen, P L., and L Levy. "Mechanism By Which Hydnocarpic Acid Inhibits Mycobacterial Multiplication." Antimicrobial Agents and Chemotherapy, vol. 3, no. 3, 1973, pp. 373-9.
Jacobsen PL, Levy L. Mechanism by which hydnocarpic acid inhibits mycobacterial multiplication. Antimicrob Agents Chemother. 1973;3(3):373-9.
Jacobsen, P. L., & Levy, L. (1973). Mechanism by which hydnocarpic acid inhibits mycobacterial multiplication. Antimicrobial Agents and Chemotherapy, 3(3), pp. 373-9.
Jacobsen PL, Levy L. Mechanism By Which Hydnocarpic Acid Inhibits Mycobacterial Multiplication. Antimicrob Agents Chemother. 1973;3(3):373-9. PubMed PMID: 4799554.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mechanism by which hydnocarpic acid inhibits mycobacterial multiplication. AU - Jacobsen,P L, AU - Levy,L, PY - 1973/3/1/pubmed PY - 1973/3/1/medline PY - 1973/3/1/entrez SP - 373 EP - 9 JF - Antimicrobial agents and chemotherapy JO - Antimicrob. Agents Chemother. VL - 3 IS - 3 N2 - Recent work in this laboratory has shown that hydnocarpic acid (HA), a principal constituent of chaulmoogra oil, inhibits multiplication in vitro of a number of mycobacterial species. This activity of HA was not shared by several straight-chain fatty acids and by dihydrochaulmoogric acid. A study of the interaction of HA with biotin has been undertaken, based on a structural analogy between biotin and the cyclopentenyl fatty acid. The multiplication of a strain of Mycobacterium intracellulare susceptible to 2 mug of HA/ml was measured turbidimetrically in Dubos medium, in the presence and absence of biotin and several other compounds. Biotin and, to a lesser extent, adenine plus guanine, palmitic acid, and linoleic acid antagonized growth inhibition by HA. Desthiobiotin, thioctic acid, and succinic acid did not block inhibition of bacterial multiplication by HA. HA may act by blocking the coenzymatic activity of biotin, or it may inhibit microbial biotin synthesis. Resumption of multiplication of M. intracellulare after a period of inhibition by HA in broth culture was found to be accompanied by reduction of the effective concentration of the drug; this could have resulted from metabolism of HA or production of an antagonist to HA by the organisms. Also, those organisms that multiplied in the presence of HA were found to represent HA-resistant mutants of M. intracellulare. SN - 0066-4804 UR - https://www.unboundmedicine.com/medline/citation/4799554/Mechanism_by_which_hydnocarpic_acid_inhibits_mycobacterial_multiplication_ L2 - http://aac.asm.org/cgi/pmidlookup?view=long&pmid=4799554 DB - PRIME DP - Unbound Medicine ER -