Tags

Type your tag names separated by a space and hit enter

Subacute sclerosing panencephalitis: propagation of measles virus from brain biopsy in tissue culture.
Science. 1969 Mar 14; 163(3872):1193-4.Sci

Abstract

Measles virus was propagated in monolayer cultures established from brain tissue of a patient with subacute sclerosing panencephalitis. Syncytial cells were rendered fluorescent with measles specific antiserums only, by means of an indirect technique. The ultrastructural appearance of the microtubular aggregates was identical in brain tissue and in the cultured cells. Fusion experiments produced a cytopathic effect in humnan embryonic kidney and VERO cell cultures. The virus was identified by hemiagglutination-inhibition, but only in the supernatant of disrupted cultured cells.

Authors

Chen TT
No affiliation info available
Watanabe I
No affiliation info available
Zeman W
No affiliation info available
Mealey J
No affiliation info available

MeSH

BrainChildCulture TechniquesCytopathogenic Effect, ViralEncephalitisFemaleFluorescent Antibody TechniqueHumansMeaslesMeasles virusMicroscopy, ElectronSclerosis

Pub Type(s)

Journal Article

Language

eng

PubMed ID

4885347

Citation

Chen, T T., et al. "Subacute Sclerosing Panencephalitis: Propagation of Measles Virus From Brain Biopsy in Tissue Culture." Science (New York, N.Y.), vol. 163, no. 3872, 1969, pp. 1193-4.
Chen TT, Watanabe I, Zeman W, et al. Subacute sclerosing panencephalitis: propagation of measles virus from brain biopsy in tissue culture. Science. 1969;163(3872):1193-4.
Chen, T. T., Watanabe, I., Zeman, W., & Mealey, J. (1969). Subacute sclerosing panencephalitis: propagation of measles virus from brain biopsy in tissue culture. Science (New York, N.Y.), 163(3872), 1193-4.
Chen TT, et al. Subacute Sclerosing Panencephalitis: Propagation of Measles Virus From Brain Biopsy in Tissue Culture. Science. 1969 Mar 14;163(3872):1193-4. PubMed PMID: 4885347.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Subacute sclerosing panencephalitis: propagation of measles virus from brain biopsy in tissue culture. AU - Chen,T T, AU - Watanabe,I, AU - Zeman,W, AU - Mealey,J,Jr PY - 1969/3/14/pubmed PY - 2001/3/28/medline PY - 1969/3/14/entrez SP - 1193 EP - 4 JF - Science (New York, N.Y.) JO - Science VL - 163 IS - 3872 N2 - Measles virus was propagated in monolayer cultures established from brain tissue of a patient with subacute sclerosing panencephalitis. Syncytial cells were rendered fluorescent with measles specific antiserums only, by means of an indirect technique. The ultrastructural appearance of the microtubular aggregates was identical in brain tissue and in the cultured cells. Fusion experiments produced a cytopathic effect in humnan embryonic kidney and VERO cell cultures. The virus was identified by hemiagglutination-inhibition, but only in the supernatant of disrupted cultured cells. SN - 0036-8075 UR - https://www.unboundmedicine.com/medline/citation/4885347/Subacute_sclerosing_panencephalitis:_propagation_of_measles_virus_from_brain_biopsy_in_tissue_culture_ DB - PRIME DP - Unbound Medicine ER -