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Synthesis and release of proinsulin and insulin by isolated rat islets of Langerhans.
J Clin Invest. 1970 Jul; 49(7):1394-404.JCI

Abstract

Isolated rat islets of Langerhans were incubated for 60, 120, and 180 min and the incorporation of leucine-(3)H into proinsulin and insulin moieties was followed. Synthesis and release of these hormones could be followed by separate extractions of islets and incubation media. RELEASE OF NEWLY SYNTHESIZED PROINSULIN AND INSULIN OCCURRED UNDER THE FOLLOWING CONDITIONS: (a) incubation for greater than 60 min; (b) glucose concentrations above 5.3 mmoles/liter; (c) incubation with 5 mM dibutyryl cyclic AMP or theophylline in 5.3 mM glucose (potentiated by 16 mM glucose); and (d) incubation with 5 mM tolbutamide and 16 mM glucose. Synthesis of proinsulin and insulin was enhanced by time of incubation, high glucose concentrations, by dibutyryl cyclic AMP or theophylline, and by tolbutamide only at 16 mM glucose. Synthesis was totally inhibited by tolbutamide at 5.3 mM glucose.

Authors

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Pub Type(s)

Journal Article

Language

eng

PubMed ID

4914679

Citation

Tanese, T, et al. "Synthesis and Release of Proinsulin and Insulin By Isolated Rat Islets of Langerhans." The Journal of Clinical Investigation, vol. 49, no. 7, 1970, pp. 1394-404.
Tanese T, Lazarus NR, Devrim S, et al. Synthesis and release of proinsulin and insulin by isolated rat islets of Langerhans. J Clin Invest. 1970;49(7):1394-404.
Tanese, T., Lazarus, N. R., Devrim, S., & Recant, L. (1970). Synthesis and release of proinsulin and insulin by isolated rat islets of Langerhans. The Journal of Clinical Investigation, 49(7), 1394-404.
Tanese T, et al. Synthesis and Release of Proinsulin and Insulin By Isolated Rat Islets of Langerhans. J Clin Invest. 1970;49(7):1394-404. PubMed PMID: 4914679.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Synthesis and release of proinsulin and insulin by isolated rat islets of Langerhans. AU - Tanese,T, AU - Lazarus,N R, AU - Devrim,S, AU - Recant,L, PY - 1970/7/1/pubmed PY - 1970/7/1/medline PY - 1970/7/1/entrez SP - 1394 EP - 404 JF - The Journal of clinical investigation JO - J Clin Invest VL - 49 IS - 7 N2 - Isolated rat islets of Langerhans were incubated for 60, 120, and 180 min and the incorporation of leucine-(3)H into proinsulin and insulin moieties was followed. Synthesis and release of these hormones could be followed by separate extractions of islets and incubation media. RELEASE OF NEWLY SYNTHESIZED PROINSULIN AND INSULIN OCCURRED UNDER THE FOLLOWING CONDITIONS: (a) incubation for greater than 60 min; (b) glucose concentrations above 5.3 mmoles/liter; (c) incubation with 5 mM dibutyryl cyclic AMP or theophylline in 5.3 mM glucose (potentiated by 16 mM glucose); and (d) incubation with 5 mM tolbutamide and 16 mM glucose. Synthesis of proinsulin and insulin was enhanced by time of incubation, high glucose concentrations, by dibutyryl cyclic AMP or theophylline, and by tolbutamide only at 16 mM glucose. Synthesis was totally inhibited by tolbutamide at 5.3 mM glucose. SN - 0021-9738 UR - https://www.unboundmedicine.com/medline/citation/4914679/Synthesis_and_release_of_proinsulin_and_insulin_by_isolated_rat_islets_of_Langerhans_ L2 - https://doi.org/10.1172/JCI106357 DB - PRIME DP - Unbound Medicine ER -