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Effect of pH changes on the binding of vitamin B12 by intrinsic factor.
J Clin Pathol. 1971 Apr; 24(3):239-43.JC

Abstract

The binding of vitamin B(12) by human gastric juice has been found to be pH dependent. Maximum binding occurs between pH 6.5 and 10. Outside this pH range the vitamin B(12)-binding ability of human gastric juice decreases and at pH below 2 or above 12.2 this drops sharply to about 10 to 15% of the maximum. Three commercial hog intrinsic factors have been found to give a similar response to pH changes. The pH-dependent binder in human gastric juice has been shown to be intrinsic factor by the addition of intrinsic factor-blocking antibody. About 10% of vitamin B(12) bound by human gastric juice is not bound by intrinsic factor and is not pH dependent. The reduction in the vitamin B(12)-binding capacity of human gastric juice induced by an adverse pH is reversed by neutralization. The physiological and clinical significance of these observations is discussed and their relevance to various procedures in vitro noted.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

5103294

Citation

Shum, H Y., et al. "Effect of pH Changes On the Binding of Vitamin B12 By Intrinsic Factor." Journal of Clinical Pathology, vol. 24, no. 3, 1971, pp. 239-43.
Shum HY, O'Neill BJ, Streeter AM. Effect of pH changes on the binding of vitamin B12 by intrinsic factor. J Clin Pathol. 1971;24(3):239-43.
Shum, H. Y., O'Neill, B. J., & Streeter, A. M. (1971). Effect of pH changes on the binding of vitamin B12 by intrinsic factor. Journal of Clinical Pathology, 24(3), 239-43.
Shum HY, O'Neill BJ, Streeter AM. Effect of pH Changes On the Binding of Vitamin B12 By Intrinsic Factor. J Clin Pathol. 1971;24(3):239-43. PubMed PMID: 5103294.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of pH changes on the binding of vitamin B12 by intrinsic factor. AU - Shum,H Y, AU - O'Neill,B J, AU - Streeter,A M, PY - 1971/4/1/pubmed PY - 1971/4/1/medline PY - 1971/4/1/entrez SP - 239 EP - 43 JF - Journal of clinical pathology JO - J Clin Pathol VL - 24 IS - 3 N2 - The binding of vitamin B(12) by human gastric juice has been found to be pH dependent. Maximum binding occurs between pH 6.5 and 10. Outside this pH range the vitamin B(12)-binding ability of human gastric juice decreases and at pH below 2 or above 12.2 this drops sharply to about 10 to 15% of the maximum. Three commercial hog intrinsic factors have been found to give a similar response to pH changes. The pH-dependent binder in human gastric juice has been shown to be intrinsic factor by the addition of intrinsic factor-blocking antibody. About 10% of vitamin B(12) bound by human gastric juice is not bound by intrinsic factor and is not pH dependent. The reduction in the vitamin B(12)-binding capacity of human gastric juice induced by an adverse pH is reversed by neutralization. The physiological and clinical significance of these observations is discussed and their relevance to various procedures in vitro noted. SN - 0021-9746 UR - https://www.unboundmedicine.com/medline/citation/5103294/Effect_of_pH_changes_on_the_binding_of_vitamin_B12_by_intrinsic_factor_ L2 - https://jcp.bmj.com/lookup/pmidlookup?view=long&pmid=5103294 DB - PRIME DP - Unbound Medicine ER -