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Development and utilization of complement-fixation and immune adherence tests for human hepatitis A virus and antibody.
Am J Med Sci 1975 Jul-Aug; 270(1):93-8AJ

Abstract

The reliable propagation of CR326 strain of human hepatitis A virus in Saguinus mystax marmosets has permitted the development of specific serum neutralization, complement-fixation (CF), and immune adherence (IA) assays for hepatitis A antigen and antibody. The CF and IA assay were made possible by the use of livers of CR326-infected marmosets as a source of hepatitis A antigen. All assays were shown to be specific for hepatitis A. Patients with hepatitis B did not show development of hepatitis A antibody. Hepatitis A antibody appeared following onset of illness, and, in the longest time period studied, has persisted for seven years. Epidemiologic studies have been performed on several Costa Rican families with outbreaks of hepatitis, with the IA and CF assays. Also, several populations in the U.S.A. were studied. These indicated a high incidence of hepatitis A at an early age in Costa Rica and a relatively low incidence of hepatitis A antibody among adults in the U.S.A. It was shown that human immune globulin can be standardized for hepatitis A antibody content by the IA assay. Finally, the IA assay indicated probable hepatitis A antibody in uninoculated chimpanzees, grivet monkeys, and rhesus monkeys.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

53013

Citation

Hilleman, M R., et al. "Development and Utilization of Complement-fixation and Immune Adherence Tests for Human Hepatitis a Virus and Antibody." The American Journal of the Medical Sciences, vol. 270, no. 1, 1975, pp. 93-8.
Hilleman MR, Provost PJ, Miller WJ, et al. Development and utilization of complement-fixation and immune adherence tests for human hepatitis A virus and antibody. Am J Med Sci. 1975;270(1):93-8.
Hilleman, M. R., Provost, P. J., Miller, W. J., Villarejos, V. M., Ittensohn, O. L., & McAleer, W. J. (1975). Development and utilization of complement-fixation and immune adherence tests for human hepatitis A virus and antibody. The American Journal of the Medical Sciences, 270(1), pp. 93-8.
Hilleman MR, et al. Development and Utilization of Complement-fixation and Immune Adherence Tests for Human Hepatitis a Virus and Antibody. Am J Med Sci. 1975;270(1):93-8. PubMed PMID: 53013.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development and utilization of complement-fixation and immune adherence tests for human hepatitis A virus and antibody. AU - Hilleman,M R, AU - Provost,P J, AU - Miller,W J, AU - Villarejos,V M, AU - Ittensohn,O L, AU - McAleer,W J, PY - 1975/7/1/pubmed PY - 1975/7/1/medline PY - 1975/7/1/entrez SP - 93 EP - 8 JF - The American journal of the medical sciences JO - Am. J. Med. Sci. VL - 270 IS - 1 N2 - The reliable propagation of CR326 strain of human hepatitis A virus in Saguinus mystax marmosets has permitted the development of specific serum neutralization, complement-fixation (CF), and immune adherence (IA) assays for hepatitis A antigen and antibody. The CF and IA assay were made possible by the use of livers of CR326-infected marmosets as a source of hepatitis A antigen. All assays were shown to be specific for hepatitis A. Patients with hepatitis B did not show development of hepatitis A antibody. Hepatitis A antibody appeared following onset of illness, and, in the longest time period studied, has persisted for seven years. Epidemiologic studies have been performed on several Costa Rican families with outbreaks of hepatitis, with the IA and CF assays. Also, several populations in the U.S.A. were studied. These indicated a high incidence of hepatitis A at an early age in Costa Rica and a relatively low incidence of hepatitis A antibody among adults in the U.S.A. It was shown that human immune globulin can be standardized for hepatitis A antibody content by the IA assay. Finally, the IA assay indicated probable hepatitis A antibody in uninoculated chimpanzees, grivet monkeys, and rhesus monkeys. SN - 0002-9629 UR - https://www.unboundmedicine.com/medline/citation/53013/Development_and_utilization_of_complement_fixation_and_immune_adherence_tests_for_human_hepatitis_A_virus_and_antibody_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=linkout&SEARCH=53013.ui DB - PRIME DP - Unbound Medicine ER -