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Development and utilization of complement-fixation and immune adherence tests for human hepatitis A virus and antibody.

Abstract

The reliable propagation of CR326 strain of human hepatitis A virus in Saguinus mystax marmosets has permitted the development of specific serum neutralization, complement-fixation (CF), and immune adherence (IA) assays for hepatitis A antigen and antibody. The CF and IA assay were made possible by the use of livers of CR326-infected marmosets as a source of hepatitis A antigen. All assays were shown to be specific for hepatitis A. Patients with hepatitis B did not show development of hepatitis A antibody. Hepatitis A antibody appeared following onset of illness, and, in the longest time period studied, has persisted for seven years. Epidemiologic studies have been performed on several Costa Rican families with outbreaks of hepatitis, with the IA and CF assays. Also, several populations in the U.S.A. were studied. These indicated a high incidence of hepatitis A at an early age in Costa Rica and a relatively low incidence of hepatitis A antibody among adults in the U.S.A. It was shown that human immune globulin can be standardized for hepatitis A antibody content by the IA assay. Finally, the IA assay indicated probable hepatitis A antibody in uninoculated chimpanzees, grivet monkeys, and rhesus monkeys.

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    MeSH

    Adolescent
    Adult
    Age Factors
    Animals
    Antibodies, Viral
    Callitrichinae
    Child
    Child, Preschool
    Complement Fixation Tests
    Costa Rica
    Cricetinae
    Epitopes
    Female
    Guinea Pigs
    Haplorhini
    Hepatitis A
    Hepatitis B
    Hepatitis B Antigens
    Hepatovirus
    Humans
    Immune Adherence Reaction
    Immune Sera
    Immunity
    Liver
    Macaca mulatta
    Male
    Pan troglodytes
    Rabbits
    Rats
    Swine
    United States

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    53013

    Citation

    Hilleman, M R., et al. "Development and Utilization of Complement-fixation and Immune Adherence Tests for Human Hepatitis a Virus and Antibody." The American Journal of the Medical Sciences, vol. 270, no. 1, 1975, pp. 93-8.
    Hilleman MR, Provost PJ, Miller WJ, et al. Development and utilization of complement-fixation and immune adherence tests for human hepatitis A virus and antibody. Am J Med Sci. 1975;270(1):93-8.
    Hilleman, M. R., Provost, P. J., Miller, W. J., Villarejos, V. M., Ittensohn, O. L., & McAleer, W. J. (1975). Development and utilization of complement-fixation and immune adherence tests for human hepatitis A virus and antibody. The American Journal of the Medical Sciences, 270(1), pp. 93-8.
    Hilleman MR, et al. Development and Utilization of Complement-fixation and Immune Adherence Tests for Human Hepatitis a Virus and Antibody. Am J Med Sci. 1975;270(1):93-8. PubMed PMID: 53013.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Development and utilization of complement-fixation and immune adherence tests for human hepatitis A virus and antibody. AU - Hilleman,M R, AU - Provost,P J, AU - Miller,W J, AU - Villarejos,V M, AU - Ittensohn,O L, AU - McAleer,W J, PY - 1975/7/1/pubmed PY - 1975/7/1/medline PY - 1975/7/1/entrez SP - 93 EP - 8 JF - The American journal of the medical sciences JO - Am. J. Med. Sci. VL - 270 IS - 1 N2 - The reliable propagation of CR326 strain of human hepatitis A virus in Saguinus mystax marmosets has permitted the development of specific serum neutralization, complement-fixation (CF), and immune adherence (IA) assays for hepatitis A antigen and antibody. The CF and IA assay were made possible by the use of livers of CR326-infected marmosets as a source of hepatitis A antigen. All assays were shown to be specific for hepatitis A. Patients with hepatitis B did not show development of hepatitis A antibody. Hepatitis A antibody appeared following onset of illness, and, in the longest time period studied, has persisted for seven years. Epidemiologic studies have been performed on several Costa Rican families with outbreaks of hepatitis, with the IA and CF assays. Also, several populations in the U.S.A. were studied. These indicated a high incidence of hepatitis A at an early age in Costa Rica and a relatively low incidence of hepatitis A antibody among adults in the U.S.A. It was shown that human immune globulin can be standardized for hepatitis A antibody content by the IA assay. Finally, the IA assay indicated probable hepatitis A antibody in uninoculated chimpanzees, grivet monkeys, and rhesus monkeys. SN - 0002-9629 UR - https://www.unboundmedicine.com/medline/citation/53013/Development_and_utilization_of_complement_fixation_and_immune_adherence_tests_for_human_hepatitis_A_virus_and_antibody_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=linkout&SEARCH=53013.ui DB - PRIME DP - Unbound Medicine ER -