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Isolation of vitamin B-12-binding proteins by combined immuno and affinity chromatography. Comparative studies on the isolated and unisolated proteins.
Biochim Biophys Acta. 1977 Dec 20; 495(2):336-48.BB

Abstract

Intrinsic factor or cobalophilin were removed by incubating human gastric juice and pig pyloric extract with purified anti-intrinsic factor and anti-cobalophilin immunoglobulin-G, respectively, covalently coupled to Sepharose. Cobalophilin (transcobalamin I) was also removed from pig serum either by using anti-cobalophilin immunoglobulin-G Sepharose or by Sephadex G-200 chromatography. The one remaining semipurified vitamin B-12-binding protein (intrinsic factor, cobalophilin or transcobalamin II) was then isolated by vitamin B-12-Sepharose affinity chromatography. Intrinsic factors, cobalophilins and transcobalamin II isolated by this two-step procedure were compared by double isotope techniques with the corresponding protein not subjected to affinity chromatography and found to be identical in reaction to antiserum, gel filtration and electrofocusing. The avidity of the isolated and unisolated intrinsic factors for the ileal intrinsic factor receptor was also the same.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

588589

Citation

Marcoullis, G, et al. "Isolation of Vitamin B-12-binding Proteins By Combined Immuno and Affinity Chromatography. Comparative Studies On the Isolated and Unisolated Proteins." Biochimica Et Biophysica Acta, vol. 495, no. 2, 1977, pp. 336-48.
Marcoullis G, Salonen EM, Gräsbeck R. Isolation of vitamin B-12-binding proteins by combined immuno and affinity chromatography. Comparative studies on the isolated and unisolated proteins. Biochim Biophys Acta. 1977;495(2):336-48.
Marcoullis, G., Salonen, E. M., & Gräsbeck, R. (1977). Isolation of vitamin B-12-binding proteins by combined immuno and affinity chromatography. Comparative studies on the isolated and unisolated proteins. Biochimica Et Biophysica Acta, 495(2), 336-48.
Marcoullis G, Salonen EM, Gräsbeck R. Isolation of Vitamin B-12-binding Proteins By Combined Immuno and Affinity Chromatography. Comparative Studies On the Isolated and Unisolated Proteins. Biochim Biophys Acta. 1977 Dec 20;495(2):336-48. PubMed PMID: 588589.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation of vitamin B-12-binding proteins by combined immuno and affinity chromatography. Comparative studies on the isolated and unisolated proteins. AU - Marcoullis,G, AU - Salonen,E M, AU - Gräsbeck,R, PY - 1977/12/20/pubmed PY - 1977/12/20/medline PY - 1977/12/20/entrez SP - 336 EP - 48 JF - Biochimica et biophysica acta JO - Biochim Biophys Acta VL - 495 IS - 2 N2 - Intrinsic factor or cobalophilin were removed by incubating human gastric juice and pig pyloric extract with purified anti-intrinsic factor and anti-cobalophilin immunoglobulin-G, respectively, covalently coupled to Sepharose. Cobalophilin (transcobalamin I) was also removed from pig serum either by using anti-cobalophilin immunoglobulin-G Sepharose or by Sephadex G-200 chromatography. The one remaining semipurified vitamin B-12-binding protein (intrinsic factor, cobalophilin or transcobalamin II) was then isolated by vitamin B-12-Sepharose affinity chromatography. Intrinsic factors, cobalophilins and transcobalamin II isolated by this two-step procedure were compared by double isotope techniques with the corresponding protein not subjected to affinity chromatography and found to be identical in reaction to antiserum, gel filtration and electrofocusing. The avidity of the isolated and unisolated intrinsic factors for the ileal intrinsic factor receptor was also the same. SN - 0006-3002 UR - https://www.unboundmedicine.com/medline/citation/588589/Isolation_of_vitamin_B_12_binding_proteins_by_combined_immuno_and_affinity_chromatography__Comparative_studies_on_the_isolated_and_unisolated_proteins_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0005-2795(77)90389-0 DB - PRIME DP - Unbound Medicine ER -