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Effect of fatty acids of omega 6 series on the biosynthesis of arachidonic acid in HTC cells.
Mol Cell Biochem. 1984 Sep; 64(1):31-7.MC

Abstract

The influence of the preincubation of HTC cells with fatty acids of omega 6 series and columbinic acid (5t, 9c, 12c 18:3) on the biosynthesis of arachidonic acid was studied. The cells were incubated on a chemically defined medium with or without the addition of unlabeled linoleic, gamma-linolenic, eicosatrienoic, arachidonic, docosatetraenoic, docosapentaenoic and columbinic acids. After 24 hr of preincubation in the presence of the aforementioned fatty acids, [1-14C]eicosa-8,11,14-trienoic acid was added to the culture medium as the only lipidic source. Twenty-four hours later the synthesis of arachidonic acid and the fatty acid composition of the cells were determined. At 20 microM concentration the omega 6 fatty acids studied except docosapentaenoic acid produced an increase on the biosynthesis of arachidonic acid compared to the cells incubated in the absence of unlabeled fatty acids in the medium. The fatty acids added to the culture medium were incorporated into the cells and modified their fatty acid composition. Columbinic acid, with a similar structure to linoleic acid, also produced a significant increase on the conversion of eicosatrienoic acid to arachidonic acid. These results would suggest that the effect of both, linoleic and columbinic acids, may be adscribed to their configuration and not necessarily to their transformation in higher homologs, since columbinic acid is unable to be desaturated.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

6092909

Citation

de Alaniz, M J., et al. "Effect of Fatty Acids of Omega 6 Series On the Biosynthesis of Arachidonic Acid in HTC Cells." Molecular and Cellular Biochemistry, vol. 64, no. 1, 1984, pp. 31-7.
de Alaniz MJ, de Gómez Dumm IN, Brenner RR. Effect of fatty acids of omega 6 series on the biosynthesis of arachidonic acid in HTC cells. Mol Cell Biochem. 1984;64(1):31-7.
de Alaniz, M. J., de Gómez Dumm, I. N., & Brenner, R. R. (1984). Effect of fatty acids of omega 6 series on the biosynthesis of arachidonic acid in HTC cells. Molecular and Cellular Biochemistry, 64(1), 31-7.
de Alaniz MJ, de Gómez Dumm IN, Brenner RR. Effect of Fatty Acids of Omega 6 Series On the Biosynthesis of Arachidonic Acid in HTC Cells. Mol Cell Biochem. 1984;64(1):31-7. PubMed PMID: 6092909.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of fatty acids of omega 6 series on the biosynthesis of arachidonic acid in HTC cells. AU - de Alaniz,M J, AU - de Gómez Dumm,I N, AU - Brenner,R R, PY - 1984/9/1/pubmed PY - 1984/9/1/medline PY - 1984/9/1/entrez SP - 31 EP - 7 JF - Molecular and cellular biochemistry JO - Mol Cell Biochem VL - 64 IS - 1 N2 - The influence of the preincubation of HTC cells with fatty acids of omega 6 series and columbinic acid (5t, 9c, 12c 18:3) on the biosynthesis of arachidonic acid was studied. The cells were incubated on a chemically defined medium with or without the addition of unlabeled linoleic, gamma-linolenic, eicosatrienoic, arachidonic, docosatetraenoic, docosapentaenoic and columbinic acids. After 24 hr of preincubation in the presence of the aforementioned fatty acids, [1-14C]eicosa-8,11,14-trienoic acid was added to the culture medium as the only lipidic source. Twenty-four hours later the synthesis of arachidonic acid and the fatty acid composition of the cells were determined. At 20 microM concentration the omega 6 fatty acids studied except docosapentaenoic acid produced an increase on the biosynthesis of arachidonic acid compared to the cells incubated in the absence of unlabeled fatty acids in the medium. The fatty acids added to the culture medium were incorporated into the cells and modified their fatty acid composition. Columbinic acid, with a similar structure to linoleic acid, also produced a significant increase on the conversion of eicosatrienoic acid to arachidonic acid. These results would suggest that the effect of both, linoleic and columbinic acids, may be adscribed to their configuration and not necessarily to their transformation in higher homologs, since columbinic acid is unable to be desaturated. SN - 0300-8177 UR - https://www.unboundmedicine.com/medline/citation/6092909/Effect_of_fatty_acids_of_omega_6_series_on_the_biosynthesis_of_arachidonic_acid_in_HTC_cells_ DB - PRIME DP - Unbound Medicine ER -