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Effect of dietary fats on arachidonic acid and eicosapentaenoic acid biosynthesis and conversion to C22 fatty acids in isolated rat liver cells.
Biochim Biophys Acta. 1984 Nov 14; 796(2):205-17.BB

Abstract

The desaturation, chain elongation and esterification of [1-14C]eicosapentaenoic acid, [1-14C]arachidonic acid, [1-14C]eicosatrienoic acid, [1-14C]linolenic acid and [1-14C]linoleic acid were studied in isolated liver cells. Rats fed diets with either 15% hydrogenated coconut oil or 15% partially hydrogenated marine oil, both deficient in essential fatty acids, 15% soybean oil or standard pellet diet with 6% fat, were used. The delta 4-desaturation of 22:5(n - 3) and 22:4(n - 6) as well as the delta 6-desaturase activity was distinctly higher in cells from animals fed coconut or marine oil than with soybean oil or standard pellet. The rate of delta 5-desaturation of 20:3(n - 6) and 20:4(n - 3) was nearly the same in cells from rats fed coconut, marine and soybean oils and higher than with standard pellet. The chain elongation of 20:5(n - 3) to 22:5(n - 3) was distinctly more pronounced than the elongation of 20:4(n - 6) with all four diets. 20:5(n - 3) was mainly esterified in the phospholipids with marine and coconut oils, and mainly in triacylglycerol with standard pellet and soybean oils. The proportion of [1-14C]20:4(n - 6) in the phospholipids to that in triacylglycerol decreased in the order marine oil greater than coconut oil greater than standard pellet greater than soybean oil. The different endogenous arachidonic acid content in the phospholipids induced by the different diets increased in the same order. 20:5(n - 3) was rapidly esterified in triacylglycerol and phospholipids, then liberated especially from the triacylglycerol fraction, chain elongated to 22:5(n - 3) and reesterified.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

6093889

Citation

Hagve, T A., and B O. Christophersen. "Effect of Dietary Fats On Arachidonic Acid and Eicosapentaenoic Acid Biosynthesis and Conversion to C22 Fatty Acids in Isolated Rat Liver Cells." Biochimica Et Biophysica Acta, vol. 796, no. 2, 1984, pp. 205-17.
Hagve TA, Christophersen BO. Effect of dietary fats on arachidonic acid and eicosapentaenoic acid biosynthesis and conversion to C22 fatty acids in isolated rat liver cells. Biochim Biophys Acta. 1984;796(2):205-17.
Hagve, T. A., & Christophersen, B. O. (1984). Effect of dietary fats on arachidonic acid and eicosapentaenoic acid biosynthesis and conversion to C22 fatty acids in isolated rat liver cells. Biochimica Et Biophysica Acta, 796(2), 205-17.
Hagve TA, Christophersen BO. Effect of Dietary Fats On Arachidonic Acid and Eicosapentaenoic Acid Biosynthesis and Conversion to C22 Fatty Acids in Isolated Rat Liver Cells. Biochim Biophys Acta. 1984 Nov 14;796(2):205-17. PubMed PMID: 6093889.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of dietary fats on arachidonic acid and eicosapentaenoic acid biosynthesis and conversion to C22 fatty acids in isolated rat liver cells. AU - Hagve,T A, AU - Christophersen,B O, PY - 1984/11/14/pubmed PY - 1984/11/14/medline PY - 1984/11/14/entrez SP - 205 EP - 17 JF - Biochimica et biophysica acta JO - Biochim Biophys Acta VL - 796 IS - 2 N2 - The desaturation, chain elongation and esterification of [1-14C]eicosapentaenoic acid, [1-14C]arachidonic acid, [1-14C]eicosatrienoic acid, [1-14C]linolenic acid and [1-14C]linoleic acid were studied in isolated liver cells. Rats fed diets with either 15% hydrogenated coconut oil or 15% partially hydrogenated marine oil, both deficient in essential fatty acids, 15% soybean oil or standard pellet diet with 6% fat, were used. The delta 4-desaturation of 22:5(n - 3) and 22:4(n - 6) as well as the delta 6-desaturase activity was distinctly higher in cells from animals fed coconut or marine oil than with soybean oil or standard pellet. The rate of delta 5-desaturation of 20:3(n - 6) and 20:4(n - 3) was nearly the same in cells from rats fed coconut, marine and soybean oils and higher than with standard pellet. The chain elongation of 20:5(n - 3) to 22:5(n - 3) was distinctly more pronounced than the elongation of 20:4(n - 6) with all four diets. 20:5(n - 3) was mainly esterified in the phospholipids with marine and coconut oils, and mainly in triacylglycerol with standard pellet and soybean oils. The proportion of [1-14C]20:4(n - 6) in the phospholipids to that in triacylglycerol decreased in the order marine oil greater than coconut oil greater than standard pellet greater than soybean oil. The different endogenous arachidonic acid content in the phospholipids induced by the different diets increased in the same order. 20:5(n - 3) was rapidly esterified in triacylglycerol and phospholipids, then liberated especially from the triacylglycerol fraction, chain elongated to 22:5(n - 3) and reesterified. SN - 0006-3002 UR - https://www.unboundmedicine.com/medline/citation/6093889/Effect_of_dietary_fats_on_arachidonic_acid_and_eicosapentaenoic_acid_biosynthesis_and_conversion_to_C22_fatty_acids_in_isolated_rat_liver_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0005-2760(84)90349-7 DB - PRIME DP - Unbound Medicine ER -