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Improved plasmid vectors for the isolation of translational lac gene fusions.
Gene 1984; 31(1-3):269-73GENE

Abstract

The beta-galactosidase fusion vector pMC1403 has been modified to include the unique cloning sites EcoRI, SmaI, BamHI, SalI, AccI, PstI and HindIII. The new vectors (pNM480, pNM481 and pNM482) allow the fusion of genes to beta-galactosidase in all three translational reading frames, and exhibit an increased sensitivity of promoter detection due to a higher copy number.

Authors

No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

6098531

Citation

Minton, N P.. "Improved Plasmid Vectors for the Isolation of Translational Lac Gene Fusions." Gene, vol. 31, no. 1-3, 1984, pp. 269-73.
Minton NP. Improved plasmid vectors for the isolation of translational lac gene fusions. Gene. 1984;31(1-3):269-73.
Minton, N. P. (1984). Improved plasmid vectors for the isolation of translational lac gene fusions. Gene, 31(1-3), pp. 269-73.
Minton NP. Improved Plasmid Vectors for the Isolation of Translational Lac Gene Fusions. Gene. 1984;31(1-3):269-73. PubMed PMID: 6098531.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Improved plasmid vectors for the isolation of translational lac gene fusions. A1 - Minton,N P, PY - 1984/11/1/pubmed PY - 1984/11/1/medline PY - 1984/11/1/entrez SP - 269 EP - 73 JF - Gene JO - Gene VL - 31 IS - 1-3 N2 - The beta-galactosidase fusion vector pMC1403 has been modified to include the unique cloning sites EcoRI, SmaI, BamHI, SalI, AccI, PstI and HindIII. The new vectors (pNM480, pNM481 and pNM482) allow the fusion of genes to beta-galactosidase in all three translational reading frames, and exhibit an increased sensitivity of promoter detection due to a higher copy number. SN - 0378-1119 UR - https://www.unboundmedicine.com/medline/citation/6098531/Improved_plasmid_vectors_for_the_isolation_of_translational_lac_gene_fusions_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0378-1119(84)90220-8 DB - PRIME DP - Unbound Medicine ER -