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[Differentiation among strains of Junín virus by intraperitoneal infection in the rat].
Rev Argent Microbiol. 1984; 16(1):11-8.RA

Abstract

The 2-day-old rat is known to resist intracerebral infection with the XJ prototype strain of Junin virus, but 95-100% mortality results when infected with the attenuated XJC13 strain. When this animal was inoculated by intraperitoneal route, behaviour was diametrically opposite: the XJ strain proved lethal, while de XJC13 led to low mortality. Studies on mortality, virus titer in different organs, and anti-viral humoral response in 2-day-old rats infected with Junin virus strains were carried out in order to use this system as a new attenuation marker. Mortality rates recorded for rats inoculated with either strain, were markedly different, being 84% in the XJ-infected group and barely reaching 17% in the XJC13 group. Brain viral titers were higher in the former group than the latter (10(5.26) PFU/ml vs. 10(3) PFU/ml at day 17 pi). For this reason, viral replication may be used as a virulence marker in this experimental model. Antibody levels were also higher in the XJ group most likely due to greater viral replication. The above findings support the use of the 2-day-old rat as a biologic attenuation marker since susceptibility to infection is strain-dependent.

Links

clones/clone libraries

Authors+Show Affiliations

Blejer JL
Cátedra de Microbiología, Parasitología e Inmunología, Facultad de Medicina, UBA, Argentina.
Lerman GD
No affiliation info available
Nejamkis MR
No affiliation info available

MeSH

AnimalsAnimals, NewbornAntibodies, ViralArenaviridaeArenaviruses, New WorldBrainDisease SusceptibilityHemorrhagic Fever, AmericanInjections, IntraperitonealLiverRatsRats, Inbred StrainsSpleenVirulence

Pub Type(s)

Comparative Study
English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

spa

PubMed ID

6101033

Citation

Blejer, J L., et al. "[Differentiation Among Strains of Junín Virus By Intraperitoneal Infection in the Rat]." Revista Argentina De Microbiologia, vol. 16, no. 1, 1984, pp. 11-8.
Blejer JL, Lerman GD, Nejamkis MR. [Differentiation among strains of Junín virus by intraperitoneal infection in the rat]. Rev Argent Microbiol. 1984;16(1):11-8.
Blejer, J. L., Lerman, G. D., & Nejamkis, M. R. (1984). [Differentiation among strains of Junín virus by intraperitoneal infection in the rat]. Revista Argentina De Microbiologia, 16(1), 11-8.
Blejer JL, Lerman GD, Nejamkis MR. [Differentiation Among Strains of Junín Virus By Intraperitoneal Infection in the Rat]. Rev Argent Microbiol. 1984;16(1):11-8. PubMed PMID: 6101033.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Differentiation among strains of Junín virus by intraperitoneal infection in the rat]. AU - Blejer,J L, AU - Lerman,G D, AU - Nejamkis,M R, PY - 1984/1/1/pubmed PY - 1984/1/1/medline PY - 1984/1/1/entrez SP - 11 EP - 8 JF - Revista Argentina de microbiologia JO - Rev Argent Microbiol VL - 16 IS - 1 N2 - The 2-day-old rat is known to resist intracerebral infection with the XJ prototype strain of Junin virus, but 95-100% mortality results when infected with the attenuated XJC13 strain. When this animal was inoculated by intraperitoneal route, behaviour was diametrically opposite: the XJ strain proved lethal, while de XJC13 led to low mortality. Studies on mortality, virus titer in different organs, and anti-viral humoral response in 2-day-old rats infected with Junin virus strains were carried out in order to use this system as a new attenuation marker. Mortality rates recorded for rats inoculated with either strain, were markedly different, being 84% in the XJ-infected group and barely reaching 17% in the XJC13 group. Brain viral titers were higher in the former group than the latter (10(5.26) PFU/ml vs. 10(3) PFU/ml at day 17 pi). For this reason, viral replication may be used as a virulence marker in this experimental model. Antibody levels were also higher in the XJ group most likely due to greater viral replication. The above findings support the use of the 2-day-old rat as a biologic attenuation marker since susceptibility to infection is strain-dependent. SN - 0325-7541 UR - https://www.unboundmedicine.com/medline/citation/6101033/[Differentiation_among_strains_of_Junín_virus_by_intraperitoneal_infection_in_the_rat]_ L2 - https://antibodies.cancer.gov/detail/CPTC-GSTP1-1 DB - PRIME DP - Unbound Medicine ER -