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[Differentiation among strains of Junín virus by intraperitoneal infection in the rat].
Rev Argent Microbiol. 1984; 16(1):11-8.RA

Abstract

The 2-day-old rat is known to resist intracerebral infection with the XJ prototype strain of Junin virus, but 95-100% mortality results when infected with the attenuated XJC13 strain. When this animal was inoculated by intraperitoneal route, behaviour was diametrically opposite: the XJ strain proved lethal, while de XJC13 led to low mortality. Studies on mortality, virus titer in different organs, and anti-viral humoral response in 2-day-old rats infected with Junin virus strains were carried out in order to use this system as a new attenuation marker. Mortality rates recorded for rats inoculated with either strain, were markedly different, being 84% in the XJ-infected group and barely reaching 17% in the XJC13 group. Brain viral titers were higher in the former group than the latter (10(5.26) PFU/ml vs. 10(3) PFU/ml at day 17 pi). For this reason, viral replication may be used as a virulence marker in this experimental model. Antibody levels were also higher in the XJ group most likely due to greater viral replication. The above findings support the use of the 2-day-old rat as a biologic attenuation marker since susceptibility to infection is strain-dependent.

Authors+Show Affiliations

Cátedra de Microbiología, Parasitología e Inmunología, Facultad de Medicina, UBA, Argentina.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

spa

PubMed ID

6101033

Citation

Blejer, J L., et al. "[Differentiation Among Strains of Junín Virus By Intraperitoneal Infection in the Rat]." Revista Argentina De Microbiologia, vol. 16, no. 1, 1984, pp. 11-8.
Blejer JL, Lerman GD, Nejamkis MR. [Differentiation among strains of Junín virus by intraperitoneal infection in the rat]. Rev Argent Microbiol. 1984;16(1):11-8.
Blejer, J. L., Lerman, G. D., & Nejamkis, M. R. (1984). [Differentiation among strains of Junín virus by intraperitoneal infection in the rat]. Revista Argentina De Microbiologia, 16(1), 11-8.
Blejer JL, Lerman GD, Nejamkis MR. [Differentiation Among Strains of Junín Virus By Intraperitoneal Infection in the Rat]. Rev Argent Microbiol. 1984;16(1):11-8. PubMed PMID: 6101033.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Differentiation among strains of Junín virus by intraperitoneal infection in the rat]. AU - Blejer,J L, AU - Lerman,G D, AU - Nejamkis,M R, PY - 1984/1/1/pubmed PY - 1984/1/1/medline PY - 1984/1/1/entrez SP - 11 EP - 8 JF - Revista Argentina de microbiologia JO - Rev Argent Microbiol VL - 16 IS - 1 N2 - The 2-day-old rat is known to resist intracerebral infection with the XJ prototype strain of Junin virus, but 95-100% mortality results when infected with the attenuated XJC13 strain. When this animal was inoculated by intraperitoneal route, behaviour was diametrically opposite: the XJ strain proved lethal, while de XJC13 led to low mortality. Studies on mortality, virus titer in different organs, and anti-viral humoral response in 2-day-old rats infected with Junin virus strains were carried out in order to use this system as a new attenuation marker. Mortality rates recorded for rats inoculated with either strain, were markedly different, being 84% in the XJ-infected group and barely reaching 17% in the XJC13 group. Brain viral titers were higher in the former group than the latter (10(5.26) PFU/ml vs. 10(3) PFU/ml at day 17 pi). For this reason, viral replication may be used as a virulence marker in this experimental model. Antibody levels were also higher in the XJ group most likely due to greater viral replication. The above findings support the use of the 2-day-old rat as a biologic attenuation marker since susceptibility to infection is strain-dependent. SN - 0325-7541 UR - https://www.unboundmedicine.com/medline/citation/6101033/[Differentiation_among_strains_of_Junín_virus_by_intraperitoneal_infection_in_the_rat]_ L2 - https://antibodies.cancer.gov/detail/CPTC-GSTP1-1 DB - PRIME DP - Unbound Medicine ER -