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Changes in localization of ouabain-sensitive, potassium-dependent p-nitrophenylphosphatase activity in human thyroid carcinoma cells.
Lab Invest. 1983 Apr; 48(4):411-8.LI

Abstract

K+-dependent, ouabain-sensitive nitrophenyl phosphatase (K+-NPPase) activity, which reflects the terminal dephosphorylation step of (Na+ + K+)-ATPase action, was studied histochemically in human thyroid normal follicular cells and in human thyroid carcinoma cells, using a newly developed one-step lead citrate method. In normal thyroid follicular cells, reaction product for K+-NPPase activity was found on the lateral plasma membrane and not on either the apical or basal plasma membrane. In thyroid carcinoma cells, a large amount of reaction product was observed on the lateral plasma membrane and also on the apical and basal plasma membrane. Appropriate control experiments indicated that the deposition of reaction product was K+ dependent and ouabain sensitive. Although there was some overlap in the distribution of reaction products for K+-NPPase and Mg2+-ATPase, significant differences were consistently observed. The biochemical findings indicated that the K+-NPPase activity per milligram of DNA in thyroid carcinoma cells was approximately 10 times higher than that in normal thyroid cells, and that a significant positive correlation exists between K+-NPPase and (Na+ + K+)-ATPase activity. The physiologic and pathologic implications of this localization for tracing the route of active Na+ transport, which might participate in the transport of iodide ion in both human thyroid normal follicular cells and human thyroid carcinoma cells, are discussed.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

6132023

Citation

Mizukami, Y, et al. "Changes in Localization of Ouabain-sensitive, Potassium-dependent P-nitrophenylphosphatase Activity in Human Thyroid Carcinoma Cells." Laboratory Investigation; a Journal of Technical Methods and Pathology, vol. 48, no. 4, 1983, pp. 411-8.
Mizukami Y, Matsubara F, Matsukawa S. Changes in localization of ouabain-sensitive, potassium-dependent p-nitrophenylphosphatase activity in human thyroid carcinoma cells. Lab Invest. 1983;48(4):411-8.
Mizukami, Y., Matsubara, F., & Matsukawa, S. (1983). Changes in localization of ouabain-sensitive, potassium-dependent p-nitrophenylphosphatase activity in human thyroid carcinoma cells. Laboratory Investigation; a Journal of Technical Methods and Pathology, 48(4), 411-8.
Mizukami Y, Matsubara F, Matsukawa S. Changes in Localization of Ouabain-sensitive, Potassium-dependent P-nitrophenylphosphatase Activity in Human Thyroid Carcinoma Cells. Lab Invest. 1983;48(4):411-8. PubMed PMID: 6132023.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Changes in localization of ouabain-sensitive, potassium-dependent p-nitrophenylphosphatase activity in human thyroid carcinoma cells. AU - Mizukami,Y, AU - Matsubara,F, AU - Matsukawa,S, PY - 1983/4/1/pubmed PY - 1983/4/1/medline PY - 1983/4/1/entrez SP - 411 EP - 8 JF - Laboratory investigation; a journal of technical methods and pathology JO - Lab Invest VL - 48 IS - 4 N2 - K+-dependent, ouabain-sensitive nitrophenyl phosphatase (K+-NPPase) activity, which reflects the terminal dephosphorylation step of (Na+ + K+)-ATPase action, was studied histochemically in human thyroid normal follicular cells and in human thyroid carcinoma cells, using a newly developed one-step lead citrate method. In normal thyroid follicular cells, reaction product for K+-NPPase activity was found on the lateral plasma membrane and not on either the apical or basal plasma membrane. In thyroid carcinoma cells, a large amount of reaction product was observed on the lateral plasma membrane and also on the apical and basal plasma membrane. Appropriate control experiments indicated that the deposition of reaction product was K+ dependent and ouabain sensitive. Although there was some overlap in the distribution of reaction products for K+-NPPase and Mg2+-ATPase, significant differences were consistently observed. The biochemical findings indicated that the K+-NPPase activity per milligram of DNA in thyroid carcinoma cells was approximately 10 times higher than that in normal thyroid cells, and that a significant positive correlation exists between K+-NPPase and (Na+ + K+)-ATPase activity. The physiologic and pathologic implications of this localization for tracing the route of active Na+ transport, which might participate in the transport of iodide ion in both human thyroid normal follicular cells and human thyroid carcinoma cells, are discussed. SN - 0023-6837 UR - https://www.unboundmedicine.com/medline/citation/6132023/Changes_in_localization_of_ouabain_sensitive_potassium_dependent_p_nitrophenylphosphatase_activity_in_human_thyroid_carcinoma_cells_ L2 - https://medlineplus.gov/thyroidcancer.html DB - PRIME DP - Unbound Medicine ER -