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Arginine infusion increases peripheral plasma somatostatin in man.
Clin Endocrinol (Oxf). 1984 Sep; 21(3):299-308.CE

Abstract

A somatostatin (SRIF) radioimmunoassay is described, using an antiserum raised in rabbit, reacting more with SRIF-14 than -28. Glass tubes were employed for the assay because our tracer, 125I 1-Tyr-SRIF, was adsorbed to plastic (23% non specific binding). Vycor extraction was used, and following Sephadex G-50 chromatography, the plasma extract showed two forms, coeluting with SRIF-28 and -14. Fifteen healthy subjects, eight women and seven men, 21-39 years old, received an infusion of arginine chloride (2.38 mmol/kg) for 20 min, or saline. An immediate rise in plasma SRIF from the mean basal level at 2 min was shown. Maximal value was 24.0 +/- 3.0 pmol/l (P less than 0.01) after 10 min followed by a rapid descent towards the basal level after the infusion. A temporal relationship was observed between the SRIF, insulin and glucagon responses following arginine infusion, while the GH levels increased only after the SRIF levels had declined, indicating an inhibition of GH. This is further supported by the high degree of correlation (r = 0.87) between SRIF- and GH increments. It is suggested that plasma SRIF measurement during arginine infusion gives an estimate of the pancreatic SRIF releasing capacity.

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Publisher Full Text

Authors

Skare S
No affiliation info available
Hanssen KF
No affiliation info available
Kriz V
No affiliation info available
Torjesen PA
No affiliation info available

MeSH

AdultArginineFemaleGlucagonGrowth HormoneHumansInsulinMaleRadioimmunoassaySomatostatinSomatostatin-28

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

6148165

Citation

Skare, S, et al. "Arginine Infusion Increases Peripheral Plasma Somatostatin in Man." Clinical Endocrinology, vol. 21, no. 3, 1984, pp. 299-308.
Skare S, Hanssen KF, Kriz V, et al. Arginine infusion increases peripheral plasma somatostatin in man. Clin Endocrinol (Oxf). 1984;21(3):299-308.
Skare, S., Hanssen, K. F., Kriz, V., & Torjesen, P. A. (1984). Arginine infusion increases peripheral plasma somatostatin in man. Clinical Endocrinology, 21(3), 299-308.
Skare S, et al. Arginine Infusion Increases Peripheral Plasma Somatostatin in Man. Clin Endocrinol (Oxf). 1984;21(3):299-308. PubMed PMID: 6148165.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Arginine infusion increases peripheral plasma somatostatin in man. AU - Skare,S, AU - Hanssen,K F, AU - Kriz,V, AU - Torjesen,P A, PY - 1984/9/1/pubmed PY - 1984/9/1/medline PY - 1984/9/1/entrez SP - 299 EP - 308 JF - Clinical endocrinology JO - Clin Endocrinol (Oxf) VL - 21 IS - 3 N2 - A somatostatin (SRIF) radioimmunoassay is described, using an antiserum raised in rabbit, reacting more with SRIF-14 than -28. Glass tubes were employed for the assay because our tracer, 125I 1-Tyr-SRIF, was adsorbed to plastic (23% non specific binding). Vycor extraction was used, and following Sephadex G-50 chromatography, the plasma extract showed two forms, coeluting with SRIF-28 and -14. Fifteen healthy subjects, eight women and seven men, 21-39 years old, received an infusion of arginine chloride (2.38 mmol/kg) for 20 min, or saline. An immediate rise in plasma SRIF from the mean basal level at 2 min was shown. Maximal value was 24.0 +/- 3.0 pmol/l (P less than 0.01) after 10 min followed by a rapid descent towards the basal level after the infusion. A temporal relationship was observed between the SRIF, insulin and glucagon responses following arginine infusion, while the GH levels increased only after the SRIF levels had declined, indicating an inhibition of GH. This is further supported by the high degree of correlation (r = 0.87) between SRIF- and GH increments. It is suggested that plasma SRIF measurement during arginine infusion gives an estimate of the pancreatic SRIF releasing capacity. SN - 0300-0664 UR - https://www.unboundmedicine.com/medline/citation/6148165/Arginine_infusion_increases_peripheral_plasma_somatostatin_in_man_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0300-0664&date=1984&volume=21&issue=3&spage=299 DB - PRIME DP - Unbound Medicine ER -