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Role of membrane bound calcium in histamine secretion from rat peritoneal mast cells.
Agents Actions. 1981 Jul; 11(4):324-9.AA

Abstract

Calcium loosely bound to the mast cell membrane may be utilized for histamine release induced by antigen, concanavalin A, compound 48/80 and the calcium ionophore A23187. Cells incubated in the presence of calcium and diluted into a medium free of divalent cations give a maximal release of histamine which decays with time, consistent with dissociation of the ion from the membrane. Anti-rat IgE and suboptimal concentrations of the ionophore show an immediate decrease in response followed by a further progressive decay. These results are consistent with the slower progress decay. These results are consistent with the slower time-course of secretion shown by these agonists, thus permitting dissociation of calcium from the membrane, before exocytosis is induced. In accord with this hypothesis, the initial suppression of response to the ionophore is reversed by preincubation of the cells with adenosine which enhances the rate of histamine release. The response of dextran is totally abolished by the described treatment, supporting suggesting that free extracellular calcium may be required for the polysaccharide to express its activity.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

6169262

Citation

White, J R., and F L. Pearce. "Role of Membrane Bound Calcium in Histamine Secretion From Rat Peritoneal Mast Cells." Agents and Actions, vol. 11, no. 4, 1981, pp. 324-9.
White JR, Pearce FL. Role of membrane bound calcium in histamine secretion from rat peritoneal mast cells. Agents Actions. 1981;11(4):324-9.
White, J. R., & Pearce, F. L. (1981). Role of membrane bound calcium in histamine secretion from rat peritoneal mast cells. Agents and Actions, 11(4), 324-9.
White JR, Pearce FL. Role of Membrane Bound Calcium in Histamine Secretion From Rat Peritoneal Mast Cells. Agents Actions. 1981;11(4):324-9. PubMed PMID: 6169262.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of membrane bound calcium in histamine secretion from rat peritoneal mast cells. AU - White,J R, AU - Pearce,F L, PY - 1981/7/1/pubmed PY - 1981/7/1/medline PY - 1981/7/1/entrez SP - 324 EP - 9 JF - Agents and actions JO - Agents Actions VL - 11 IS - 4 N2 - Calcium loosely bound to the mast cell membrane may be utilized for histamine release induced by antigen, concanavalin A, compound 48/80 and the calcium ionophore A23187. Cells incubated in the presence of calcium and diluted into a medium free of divalent cations give a maximal release of histamine which decays with time, consistent with dissociation of the ion from the membrane. Anti-rat IgE and suboptimal concentrations of the ionophore show an immediate decrease in response followed by a further progressive decay. These results are consistent with the slower progress decay. These results are consistent with the slower time-course of secretion shown by these agonists, thus permitting dissociation of calcium from the membrane, before exocytosis is induced. In accord with this hypothesis, the initial suppression of response to the ionophore is reversed by preincubation of the cells with adenosine which enhances the rate of histamine release. The response of dextran is totally abolished by the described treatment, supporting suggesting that free extracellular calcium may be required for the polysaccharide to express its activity. SN - 0065-4299 UR - https://www.unboundmedicine.com/medline/citation/6169262/Role_of_membrane_bound_calcium_in_histamine_secretion_from_rat_peritoneal_mast_cells_ DB - PRIME DP - Unbound Medicine ER -