Tags

Type your tag names separated by a space and hit enter

Characterization of antigenic determinants on human myeloid colony forming cells with monoclonal antibodies.
Exp Hematol. 1981 Aug; 9(7):781-7.EH

Abstract

Immunologic characterization of myeloid progenitor cells (CFUGM) provides a new dimension for identification and separation of this hemopoietic cell population from other cells within marrow and peripheral blood. Monoclonal antibodies against human anti Ia-like (HLA-DR) determinants and against T lymphocytes were utilized to more precisely define the cell surface antigenic structure of human CFUGM. Complement-mediated cytotoxicity testing demonstrated the presence of HLA-DR antigens and absence of a T lymphocyte antigen on the clonogenic CFUGM. Similar degrees of cytotoxicity were noted for B lymphocytes and CFUGM using anti HLA-DR monoclonal antibodies. Our studies with the anti T lymphocyte antibody suggest that T lymphocytes may be selectively removed from marrow cells without depletion of myeloid precursor cells.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

6172283

Citation

Greenberg, P, et al. "Characterization of Antigenic Determinants On Human Myeloid Colony Forming Cells With Monoclonal Antibodies." Experimental Hematology, vol. 9, no. 7, 1981, pp. 781-7.
Greenberg P, Grossman M, Charron D, et al. Characterization of antigenic determinants on human myeloid colony forming cells with monoclonal antibodies. Exp Hematol. 1981;9(7):781-7.
Greenberg, P., Grossman, M., Charron, D., & Levy, R. (1981). Characterization of antigenic determinants on human myeloid colony forming cells with monoclonal antibodies. Experimental Hematology, 9(7), 781-7.
Greenberg P, et al. Characterization of Antigenic Determinants On Human Myeloid Colony Forming Cells With Monoclonal Antibodies. Exp Hematol. 1981;9(7):781-7. PubMed PMID: 6172283.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of antigenic determinants on human myeloid colony forming cells with monoclonal antibodies. AU - Greenberg,P, AU - Grossman,M, AU - Charron,D, AU - Levy,R, PY - 1981/8/1/pubmed PY - 1981/8/1/medline PY - 1981/8/1/entrez SP - 781 EP - 7 JF - Experimental hematology JO - Exp Hematol VL - 9 IS - 7 N2 - Immunologic characterization of myeloid progenitor cells (CFUGM) provides a new dimension for identification and separation of this hemopoietic cell population from other cells within marrow and peripheral blood. Monoclonal antibodies against human anti Ia-like (HLA-DR) determinants and against T lymphocytes were utilized to more precisely define the cell surface antigenic structure of human CFUGM. Complement-mediated cytotoxicity testing demonstrated the presence of HLA-DR antigens and absence of a T lymphocyte antigen on the clonogenic CFUGM. Similar degrees of cytotoxicity were noted for B lymphocytes and CFUGM using anti HLA-DR monoclonal antibodies. Our studies with the anti T lymphocyte antibody suggest that T lymphocytes may be selectively removed from marrow cells without depletion of myeloid precursor cells. SN - 0301-472X UR - https://www.unboundmedicine.com/medline/citation/6172283/Characterization_of_antigenic_determinants_on_human_myeloid_colony_forming_cells_with_monoclonal_antibodies_ L2 - https://medlineplus.gov/stemcells.html DB - PRIME DP - Unbound Medicine ER -