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Office laboratory identification of Neisseria gonorrhoeae.
J Fam Pract. 1982 Jan; 14(1):35-7.JF

Abstract

Current literature is contradictory regarding what tests are necessary to establish an adequate presumptive diagnosis for office identification of Neisseria gonorrhoeae. This is especially true in light of recent reports of N meningitidis causing acute genital infection. This study was designed to look at the various criteria recommended and to establish guidelines for the office identification of N gonorrhoeae. Four hundred thirty-six isolates grown on Thayer-Martin selective agar were studied. Of these, 156 were found to be oxidase-positive. Gram stain of the isolates showed that 31.4 percent of the oxidase-positive isolates were gram-negative diplococci, 59 percent were yeastlike mole, and 9.7 percent were gram-negative bacilli. All of the gram-negative diplococci were confirmed to be N gonorrhoeae by sugar fermentation studies. No isolates of N meningitidis or saprophytic Neisseria were found. On the basis of this finding, it is recommended that office identification of N gonorrhoeae from genital or anal cultures should include (1) growth on Thayer-Martin (or comparable) medium, (2) positive reaction to oxidase reagent, and (3) identification of gram-negative diplococci on Gram stain of the Thayer-Martin isolate.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

6172543

Citation

Waldman, C R., et al. "Office Laboratory Identification of Neisseria Gonorrhoeae." The Journal of Family Practice, vol. 14, no. 1, 1982, pp. 35-7.
Waldman CR, Gaydos JM, Synder FF. Office laboratory identification of Neisseria gonorrhoeae. J Fam Pract. 1982;14(1):35-7.
Waldman, C. R., Gaydos, J. M., & Synder, F. F. (1982). Office laboratory identification of Neisseria gonorrhoeae. The Journal of Family Practice, 14(1), 35-7.
Waldman CR, Gaydos JM, Synder FF. Office Laboratory Identification of Neisseria Gonorrhoeae. J Fam Pract. 1982;14(1):35-7. PubMed PMID: 6172543.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Office laboratory identification of Neisseria gonorrhoeae. AU - Waldman,C R, AU - Gaydos,J M, AU - Synder,F F, PY - 1982/1/1/pubmed PY - 1982/1/1/medline PY - 1982/1/1/entrez SP - 35 EP - 7 JF - The Journal of family practice JO - J Fam Pract VL - 14 IS - 1 N2 - Current literature is contradictory regarding what tests are necessary to establish an adequate presumptive diagnosis for office identification of Neisseria gonorrhoeae. This is especially true in light of recent reports of N meningitidis causing acute genital infection. This study was designed to look at the various criteria recommended and to establish guidelines for the office identification of N gonorrhoeae. Four hundred thirty-six isolates grown on Thayer-Martin selective agar were studied. Of these, 156 were found to be oxidase-positive. Gram stain of the isolates showed that 31.4 percent of the oxidase-positive isolates were gram-negative diplococci, 59 percent were yeastlike mole, and 9.7 percent were gram-negative bacilli. All of the gram-negative diplococci were confirmed to be N gonorrhoeae by sugar fermentation studies. No isolates of N meningitidis or saprophytic Neisseria were found. On the basis of this finding, it is recommended that office identification of N gonorrhoeae from genital or anal cultures should include (1) growth on Thayer-Martin (or comparable) medium, (2) positive reaction to oxidase reagent, and (3) identification of gram-negative diplococci on Gram stain of the Thayer-Martin isolate. SN - 0094-3509 UR - https://www.unboundmedicine.com/medline/citation/6172543/Office_laboratory_identification_of_Neisseria_gonorrhoeae_ L2 - https://medlineplus.gov/healthfacilities.html DB - PRIME DP - Unbound Medicine ER -