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Functional differences between rat islets of ventral and dorsal pancreatic origin.
J Clin Invest. 1982 Feb; 69(2):405-13.JCI

Abstract

Do functional linkages between islet endocrine cells exist? The effect of differences in frequency and distribution of islet endocrine cells on B cell function was examined in islets from the ventral (ventral islets) and dorsal (dorsal islets) areas of the rat pancreas. Dorsal islets contained 10 times as much glucagon as ventral islets, whereas insulin and total protein contents were similar. Basal rates of insulin secretion and proinsulin biosynthesis were similar in the two types of islet, but, under conditions of glucose stimulation, both insulin secretion and proinsulin biosynthesis were significantly greater in the glucagon-rich dorsal islets. Similarly, glucose utilization rates an ATP levels were greater in dorsal islets. In contrast, the rates of processing of newly synthesized proinsulin were similar in ventral and dorsal islets. That the islet glucagon content may have affected B cell function is inferred from two independent findings. Firstly, basal and glucose-stimulated cyclic AMP contents of glucagon-rich dorsal islets were greater than those of ventral islets. Secondly, in the presence of excess exogenous glucagon (1 microgram/ml), the differences in glucose-induced insulin secretion and proinsulin biosynthesis rates between the two types of islets were eliminated. These results strongly suggest that changes in the relative proportions of the different islet endocrine cells exert marked effects on islet function. In particular, a greater A cell and glucagon content is associated with higher rates of glucose-induced insulin secretion and biosynthesis.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

6173398

Citation

Trimble, E R., et al. "Functional Differences Between Rat Islets of Ventral and Dorsal Pancreatic Origin." The Journal of Clinical Investigation, vol. 69, no. 2, 1982, pp. 405-13.
Trimble ER, Halban PA, Wollheim CB, et al. Functional differences between rat islets of ventral and dorsal pancreatic origin. J Clin Invest. 1982;69(2):405-13.
Trimble, E. R., Halban, P. A., Wollheim, C. B., & Renold, A. E. (1982). Functional differences between rat islets of ventral and dorsal pancreatic origin. The Journal of Clinical Investigation, 69(2), 405-13.
Trimble ER, et al. Functional Differences Between Rat Islets of Ventral and Dorsal Pancreatic Origin. J Clin Invest. 1982;69(2):405-13. PubMed PMID: 6173398.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Functional differences between rat islets of ventral and dorsal pancreatic origin. AU - Trimble,E R, AU - Halban,P A, AU - Wollheim,C B, AU - Renold,A E, PY - 1982/2/1/pubmed PY - 1982/2/1/medline PY - 1982/2/1/entrez SP - 405 EP - 13 JF - The Journal of clinical investigation JO - J Clin Invest VL - 69 IS - 2 N2 - Do functional linkages between islet endocrine cells exist? The effect of differences in frequency and distribution of islet endocrine cells on B cell function was examined in islets from the ventral (ventral islets) and dorsal (dorsal islets) areas of the rat pancreas. Dorsal islets contained 10 times as much glucagon as ventral islets, whereas insulin and total protein contents were similar. Basal rates of insulin secretion and proinsulin biosynthesis were similar in the two types of islet, but, under conditions of glucose stimulation, both insulin secretion and proinsulin biosynthesis were significantly greater in the glucagon-rich dorsal islets. Similarly, glucose utilization rates an ATP levels were greater in dorsal islets. In contrast, the rates of processing of newly synthesized proinsulin were similar in ventral and dorsal islets. That the islet glucagon content may have affected B cell function is inferred from two independent findings. Firstly, basal and glucose-stimulated cyclic AMP contents of glucagon-rich dorsal islets were greater than those of ventral islets. Secondly, in the presence of excess exogenous glucagon (1 microgram/ml), the differences in glucose-induced insulin secretion and proinsulin biosynthesis rates between the two types of islets were eliminated. These results strongly suggest that changes in the relative proportions of the different islet endocrine cells exert marked effects on islet function. In particular, a greater A cell and glucagon content is associated with higher rates of glucose-induced insulin secretion and biosynthesis. SN - 0021-9738 UR - https://www.unboundmedicine.com/medline/citation/6173398/Functional_differences_between_rat_islets_of_ventral_and_dorsal_pancreatic_origin_ L2 - https://doi.org/10.1172/jci110464 DB - PRIME DP - Unbound Medicine ER -