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Release of leukotrienes from guinea pig lung stimulated by C5ades Arg anaphylatoxin.
J Immunol. 1982 May; 128(5):2247-52.JI

Abstract

The complement anaphylatoxins C5a and C5Ades Arg contract guinea pig peripheral airway preparations and trachea by a mechanism largely independent of histamine release. In trachea the contractions are inhibited by FPL 55712, a relatively specific inhibitor of slow-reacting substance of anaphylaxis (SRS-A). SRS-A is now known to be a mixture of leukotrienes C4, D4, and E4 (LTC4, LTD4, LTE4). These data suggest that C5-derived anaphylatoxins stimulate production and release of leukotrienes in pulmonary tissues. To define these observations more precisely, fragments of guinea pig lung were incubated with porcine C5ades Arg, and the supernatant fluids were analyzed for leukotrienes by using both pharmacologic and chemical methods. In addition to histamine, a smooth muscle contracting activity characteristic of SRS-A was released from C5a-treated lung preparations. The contractile substance was identified as a leukotriene based on: 1) the characteristic contraction of guinea pig ileum, 2) inhibition of the contractile activity by FPL 55712, 3) enhanced release of activity in the presence of indomethacin or L-cysteine, 4) chromatographic behavior of ethanol-extracted active material on Amberlite XAD-7 resin, and 5) cochromatography of the active material on reverse-phase, high performance liquid chromatography with standard LTD4. We therefore concluded the humoral factor C5ades Arg induces a leukotriene release reaction in guinea pig lung tissue. This particular response of pulmonary tissue to anaphylatoxin has not been appreciated previously as an immediate effect of complement activation on the pathophysiology of the lung.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

6174625

Citation

Stimler, N P., et al. "Release of Leukotrienes From Guinea Pig Lung Stimulated By C5ades Arg Anaphylatoxin." Journal of Immunology (Baltimore, Md. : 1950), vol. 128, no. 5, 1982, pp. 2247-52.
Stimler NP, Bach MK, Bloor CM, et al. Release of leukotrienes from guinea pig lung stimulated by C5ades Arg anaphylatoxin. J Immunol. 1982;128(5):2247-52.
Stimler, N. P., Bach, M. K., Bloor, C. M., & Hugli, T. E. (1982). Release of leukotrienes from guinea pig lung stimulated by C5ades Arg anaphylatoxin. Journal of Immunology (Baltimore, Md. : 1950), 128(5), 2247-52.
Stimler NP, et al. Release of Leukotrienes From Guinea Pig Lung Stimulated By C5ades Arg Anaphylatoxin. J Immunol. 1982;128(5):2247-52. PubMed PMID: 6174625.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Release of leukotrienes from guinea pig lung stimulated by C5ades Arg anaphylatoxin. AU - Stimler,N P, AU - Bach,M K, AU - Bloor,C M, AU - Hugli,T E, PY - 1982/5/1/pubmed PY - 1982/5/1/medline PY - 1982/5/1/entrez SP - 2247 EP - 52 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 128 IS - 5 N2 - The complement anaphylatoxins C5a and C5Ades Arg contract guinea pig peripheral airway preparations and trachea by a mechanism largely independent of histamine release. In trachea the contractions are inhibited by FPL 55712, a relatively specific inhibitor of slow-reacting substance of anaphylaxis (SRS-A). SRS-A is now known to be a mixture of leukotrienes C4, D4, and E4 (LTC4, LTD4, LTE4). These data suggest that C5-derived anaphylatoxins stimulate production and release of leukotrienes in pulmonary tissues. To define these observations more precisely, fragments of guinea pig lung were incubated with porcine C5ades Arg, and the supernatant fluids were analyzed for leukotrienes by using both pharmacologic and chemical methods. In addition to histamine, a smooth muscle contracting activity characteristic of SRS-A was released from C5a-treated lung preparations. The contractile substance was identified as a leukotriene based on: 1) the characteristic contraction of guinea pig ileum, 2) inhibition of the contractile activity by FPL 55712, 3) enhanced release of activity in the presence of indomethacin or L-cysteine, 4) chromatographic behavior of ethanol-extracted active material on Amberlite XAD-7 resin, and 5) cochromatography of the active material on reverse-phase, high performance liquid chromatography with standard LTD4. We therefore concluded the humoral factor C5ades Arg induces a leukotriene release reaction in guinea pig lung tissue. This particular response of pulmonary tissue to anaphylatoxin has not been appreciated previously as an immediate effect of complement activation on the pathophysiology of the lung. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/6174625/Release_of_leukotrienes_from_guinea_pig_lung_stimulated_by_C5ades_Arg_anaphylatoxin_ DB - PRIME DP - Unbound Medicine ER -