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Isolation and properties of mast cells from the small bowel lamina propria of the rat.
Agents Actions. 1982 Apr; 12(1-2):183-5.AA

Abstract

A method is described for the isolation of mast cells from the mucosa of the small intestine of rats infected with the nematode Nippostrongylus brasiliensis. The cells release histamine on challenge with IgE-directed ligands and calcium ionophores but, in contrast to rat peritoneal mast cells, are refractory to the action of basic secretagogues. The mucosal and peritoneal cells also differ markedly in their sensitivity to particular anti-allergic drugs. These results further emphasize the functional heterogeneity of mast cells from different sources.

Authors

Pearce FL
No affiliation info available
Befus AD
No affiliation info available
Bienenstock J
No affiliation info available

MeSH

AnaphylaxisAnimalsCalcimycinCell SeparationHistamineHistamine ReleaseHookworm InfectionsImmunoglobulin EIn Vitro TechniquesIntestinal Diseases, ParasiticIntestine, SmallMast CellsNippostrongylusRatsp-Methoxy-N-methylphenethylamine

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

6177212

Citation

Pearce, F L., et al. "Isolation and Properties of Mast Cells From the Small Bowel Lamina Propria of the Rat." Agents and Actions, vol. 12, no. 1-2, 1982, pp. 183-5.
Pearce FL, Befus AD, Bienenstock J. Isolation and properties of mast cells from the small bowel lamina propria of the rat. Agents Actions. 1982;12(1-2):183-5.
Pearce, F. L., Befus, A. D., & Bienenstock, J. (1982). Isolation and properties of mast cells from the small bowel lamina propria of the rat. Agents and Actions, 12(1-2), 183-5.
Pearce FL, Befus AD, Bienenstock J. Isolation and Properties of Mast Cells From the Small Bowel Lamina Propria of the Rat. Agents Actions. 1982;12(1-2):183-5. PubMed PMID: 6177212.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation and properties of mast cells from the small bowel lamina propria of the rat. AU - Pearce,F L, AU - Befus,A D, AU - Bienenstock,J, PY - 1982/4/1/pubmed PY - 1982/4/1/medline PY - 1982/4/1/entrez SP - 183 EP - 5 JF - Agents and actions JO - Agents Actions VL - 12 IS - 1-2 N2 - A method is described for the isolation of mast cells from the mucosa of the small intestine of rats infected with the nematode Nippostrongylus brasiliensis. The cells release histamine on challenge with IgE-directed ligands and calcium ionophores but, in contrast to rat peritoneal mast cells, are refractory to the action of basic secretagogues. The mucosal and peritoneal cells also differ markedly in their sensitivity to particular anti-allergic drugs. These results further emphasize the functional heterogeneity of mast cells from different sources. SN - 0065-4299 UR - https://www.unboundmedicine.com/medline/citation/6177212/Isolation_and_properties_of_mast_cells_from_the_small_bowel_lamina_propria_of_the_rat_ DB - PRIME DP - Unbound Medicine ER -