Tags

Type your tag names separated by a space and hit enter

Autoimmune effector cells. III. Role of adjuvant and accessory cells in the in vitro induction of autoimmune encephalomyelitis.
J Immunol. 1982 Aug; 129(2):759-63.JI

Abstract

The present investigation shows that autoreactive effector cells that transfer experimental allergic encephalomyelitis (EAE) can be activated from spleens and lymph nodes of Lewis rats given a single injection of 25 micrograms myelin basic protein (BP) in incomplete Freund's adjuvant (IFA), despite the fact that the cell donors do not develop EAE. Rather, these donor rats are unresponsive to EAE when given an encephalitogenic emulsion of BP in complete Freund's adjuvant (CFA). Lymphoid cells from rats given a single injection of BP-IFA were almost as effective as cells from BP-CFA-treated rats with respect to transferring EAE after in vitro activation with BP or concanavalin A (Con A). Irrespective of whether donors received BP in IFA or CFA, BP-cultured spleen and lymph node cells (SpC and LNC, respectively) transferred EAE, whereas Con A-cultured SpC but not LNC exhibited effector cell activity. Con A-cultured LNC were able to transfer EAE if the cultures were reconstituted with irradiated adherent phagocytic cells (which could be obtained from normal Lewis rat spleens) or with conditioned medium from these adherent SpC. These findings indicate that accessory cells are required for in vitro induction of this T cell-mediated autoimmune response.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

6177781

Citation

Killen, J A., and R H. Swanborg. "Autoimmune Effector Cells. III. Role of Adjuvant and Accessory Cells in the in Vitro Induction of Autoimmune Encephalomyelitis." Journal of Immunology (Baltimore, Md. : 1950), vol. 129, no. 2, 1982, pp. 759-63.
Killen JA, Swanborg RH. Autoimmune effector cells. III. Role of adjuvant and accessory cells in the in vitro induction of autoimmune encephalomyelitis. J Immunol. 1982;129(2):759-63.
Killen, J. A., & Swanborg, R. H. (1982). Autoimmune effector cells. III. Role of adjuvant and accessory cells in the in vitro induction of autoimmune encephalomyelitis. Journal of Immunology (Baltimore, Md. : 1950), 129(2), 759-63.
Killen JA, Swanborg RH. Autoimmune Effector Cells. III. Role of Adjuvant and Accessory Cells in the in Vitro Induction of Autoimmune Encephalomyelitis. J Immunol. 1982;129(2):759-63. PubMed PMID: 6177781.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Autoimmune effector cells. III. Role of adjuvant and accessory cells in the in vitro induction of autoimmune encephalomyelitis. AU - Killen,J A, AU - Swanborg,R H, PY - 1982/8/1/pubmed PY - 1982/8/1/medline PY - 1982/8/1/entrez SP - 759 EP - 63 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 129 IS - 2 N2 - The present investigation shows that autoreactive effector cells that transfer experimental allergic encephalomyelitis (EAE) can be activated from spleens and lymph nodes of Lewis rats given a single injection of 25 micrograms myelin basic protein (BP) in incomplete Freund's adjuvant (IFA), despite the fact that the cell donors do not develop EAE. Rather, these donor rats are unresponsive to EAE when given an encephalitogenic emulsion of BP in complete Freund's adjuvant (CFA). Lymphoid cells from rats given a single injection of BP-IFA were almost as effective as cells from BP-CFA-treated rats with respect to transferring EAE after in vitro activation with BP or concanavalin A (Con A). Irrespective of whether donors received BP in IFA or CFA, BP-cultured spleen and lymph node cells (SpC and LNC, respectively) transferred EAE, whereas Con A-cultured SpC but not LNC exhibited effector cell activity. Con A-cultured LNC were able to transfer EAE if the cultures were reconstituted with irradiated adherent phagocytic cells (which could be obtained from normal Lewis rat spleens) or with conditioned medium from these adherent SpC. These findings indicate that accessory cells are required for in vitro induction of this T cell-mediated autoimmune response. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/6177781/Autoimmune_effector_cells__III__Role_of_adjuvant_and_accessory_cells_in_the_in_vitro_induction_of_autoimmune_encephalomyelitis_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=6177781 DB - PRIME DP - Unbound Medicine ER -