Tags

Type your tag names separated by a space and hit enter

SDS-PAGE analysis of M. leprae protein antigens reacting with antibodies from sera from lepromatous patients and infected armadillos.
Clin Exp Immunol. 1982 Sep; 49(3):523-31.CE

Abstract

Studies have been conducted to characterize M. leprae bacilli derived from infected armadillos. First, the proteins of the mycobacterial extracts were fractionated by SDS-PAGE. Subsequently, the proteins in the gel were electrophoretically transferred on a strip of nitrocellulose paper by the technique of 'electrophoretic blotting'. The separated bacterial protein bands, thus immobilized on the nitrocellulose paper were made to react immunologically with sera from the lepromatous patients, infected armadillo sera and other experimental mycobacterial antisera. It was observed that a majority of M. leprae proteins contained antigenic determinants also present on proteins of BCG. In addition, only two specific antigen bands of 33KD and 12KD were conspicuously detected by the patients' sera and the infected armadillo sera. These substances were further identified as polysaccharides or glycoproteins since they could only be stained by Schiff's reagent or alcian blue. Only 12KD glycoprotein band reacted with concanavalin A, whereas wheat germ agglutinin (WGA) did not show any reaction with them. These 33KD and 12KD glycoprotein antigens were found to lose their antigenicity after pepsin treatment and can be considered as glycoproteins. Further, radiolabelling experiments showed that 12KD antigen underwent radioiodination under usual conditions, but 33KD glycoprotein failed to be similarly radiolabelled. It is suggested that these protein antigens have M. leprae specific determinants on a cross-reacting component.

Links

PMC Free PDF
PMC Free Full Text

Authors

Chakrabarty AK
No affiliation info available
Maire MA
No affiliation info available
Lambert PH
No affiliation info available

MeSH

AnimalsAntibodies, BacterialAntigens, BacterialArmadillosBacterial ProteinsCingulataElectrophoresis, Polyacrylamide GelEpitopesGlycoproteinsHumansLeprosyMycobacterium leprae

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

6184189

Citation

Chakrabarty, A K., et al. "SDS-PAGE Analysis of M. Leprae Protein Antigens Reacting With Antibodies From Sera From Lepromatous Patients and Infected Armadillos." Clinical and Experimental Immunology, vol. 49, no. 3, 1982, pp. 523-31.
Chakrabarty AK, Maire MA, Lambert PH. SDS-PAGE analysis of M. leprae protein antigens reacting with antibodies from sera from lepromatous patients and infected armadillos. Clin Exp Immunol. 1982;49(3):523-31.
Chakrabarty, A. K., Maire, M. A., & Lambert, P. H. (1982). SDS-PAGE analysis of M. leprae protein antigens reacting with antibodies from sera from lepromatous patients and infected armadillos. Clinical and Experimental Immunology, 49(3), 523-31.
Chakrabarty AK, Maire MA, Lambert PH. SDS-PAGE Analysis of M. Leprae Protein Antigens Reacting With Antibodies From Sera From Lepromatous Patients and Infected Armadillos. Clin Exp Immunol. 1982;49(3):523-31. PubMed PMID: 6184189.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - SDS-PAGE analysis of M. leprae protein antigens reacting with antibodies from sera from lepromatous patients and infected armadillos. AU - Chakrabarty,A K, AU - Maire,M A, AU - Lambert,P H, PY - 1982/9/1/pubmed PY - 1982/9/1/medline PY - 1982/9/1/entrez SP - 523 EP - 31 JF - Clinical and experimental immunology JO - Clin Exp Immunol VL - 49 IS - 3 N2 - Studies have been conducted to characterize M. leprae bacilli derived from infected armadillos. First, the proteins of the mycobacterial extracts were fractionated by SDS-PAGE. Subsequently, the proteins in the gel were electrophoretically transferred on a strip of nitrocellulose paper by the technique of 'electrophoretic blotting'. The separated bacterial protein bands, thus immobilized on the nitrocellulose paper were made to react immunologically with sera from the lepromatous patients, infected armadillo sera and other experimental mycobacterial antisera. It was observed that a majority of M. leprae proteins contained antigenic determinants also present on proteins of BCG. In addition, only two specific antigen bands of 33KD and 12KD were conspicuously detected by the patients' sera and the infected armadillo sera. These substances were further identified as polysaccharides or glycoproteins since they could only be stained by Schiff's reagent or alcian blue. Only 12KD glycoprotein band reacted with concanavalin A, whereas wheat germ agglutinin (WGA) did not show any reaction with them. These 33KD and 12KD glycoprotein antigens were found to lose their antigenicity after pepsin treatment and can be considered as glycoproteins. Further, radiolabelling experiments showed that 12KD antigen underwent radioiodination under usual conditions, but 33KD glycoprotein failed to be similarly radiolabelled. It is suggested that these protein antigens have M. leprae specific determinants on a cross-reacting component. SN - 0009-9104 UR - https://www.unboundmedicine.com/medline/citation/6184189/SDS_PAGE_analysis_of_M__leprae_protein_antigens_reacting_with_antibodies_from_sera_from_lepromatous_patients_and_infected_armadillos_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/6184189/ DB - PRIME DP - Unbound Medicine ER -