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Antigen-presenting capabilities of human monocytes correlates with their expression of HLA-DS, an Ia determinant distinct from HLA-DR.
J Immunol. 1983 Feb; 130(2):706-11.JI

Abstract

Utilizing a monoclonal antibody (Mac-120) specific for 40 to 60% of peripheral blood adherent mononuclear cells (M phi), we were able to separate M phi into two populations based on their reactivity with the antibody. Both populations, Mac-120+ and Mac-120- cells, were then compared for a) their ability to present antigen to T cells, b) their display of HLA-DR determinants, c) their ability to stimulate in an autologous and allogeneic mixed lymphocyte reaction, and d) their display of an Ia molecule, HLA-DS, which is distinct from HLA-DR and which is homologous with murine I-A. Our findings indicate that a) only Mac-120+ cells can present antigen, b) Mac-120+ and Mac-120- cell populations are equivalent in terms of the number of HLA-DR+ cells and in the mean density of HLA-DR determinants per cell, c) although Mac-120+ and Mac-120- cells are equivalent in their ability to serve as stimulators in an allogeneic mixed lymphocyte reaction, Mac-120+ cells are better stimulators in an autologous mixed lymphocyte reaction, and d) only Mac-120+ cells display HLA-DS. These studies demonstrate that peripheral adherent mononuclear cells exhibit heterogeneity with regard to their display of Ia antigens. Furthermore, they provide functional data to support the existence of a human Ia determinant, HLA-DS, which is distinct from HLA-DR and which is important in antigen presentation and stimulation in the autologous mixed lymphocyte reaction.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

6184401

Citation

Gonwa, T A., et al. "Antigen-presenting Capabilities of Human Monocytes Correlates With Their Expression of HLA-DS, an Ia Determinant Distinct From HLA-DR." Journal of Immunology (Baltimore, Md. : 1950), vol. 130, no. 2, 1983, pp. 706-11.
Gonwa TA, Picker LJ, Raff HV, et al. Antigen-presenting capabilities of human monocytes correlates with their expression of HLA-DS, an Ia determinant distinct from HLA-DR. J Immunol. 1983;130(2):706-11.
Gonwa, T. A., Picker, L. J., Raff, H. V., Goyert, S. M., Silver, J., & Stobo, J. D. (1983). Antigen-presenting capabilities of human monocytes correlates with their expression of HLA-DS, an Ia determinant distinct from HLA-DR. Journal of Immunology (Baltimore, Md. : 1950), 130(2), 706-11.
Gonwa TA, et al. Antigen-presenting Capabilities of Human Monocytes Correlates With Their Expression of HLA-DS, an Ia Determinant Distinct From HLA-DR. J Immunol. 1983;130(2):706-11. PubMed PMID: 6184401.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antigen-presenting capabilities of human monocytes correlates with their expression of HLA-DS, an Ia determinant distinct from HLA-DR. AU - Gonwa,T A, AU - Picker,L J, AU - Raff,H V, AU - Goyert,S M, AU - Silver,J, AU - Stobo,J D, PY - 1983/2/1/pubmed PY - 1983/2/1/medline PY - 1983/2/1/entrez SP - 706 EP - 11 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 130 IS - 2 N2 - Utilizing a monoclonal antibody (Mac-120) specific for 40 to 60% of peripheral blood adherent mononuclear cells (M phi), we were able to separate M phi into two populations based on their reactivity with the antibody. Both populations, Mac-120+ and Mac-120- cells, were then compared for a) their ability to present antigen to T cells, b) their display of HLA-DR determinants, c) their ability to stimulate in an autologous and allogeneic mixed lymphocyte reaction, and d) their display of an Ia molecule, HLA-DS, which is distinct from HLA-DR and which is homologous with murine I-A. Our findings indicate that a) only Mac-120+ cells can present antigen, b) Mac-120+ and Mac-120- cell populations are equivalent in terms of the number of HLA-DR+ cells and in the mean density of HLA-DR determinants per cell, c) although Mac-120+ and Mac-120- cells are equivalent in their ability to serve as stimulators in an allogeneic mixed lymphocyte reaction, Mac-120+ cells are better stimulators in an autologous mixed lymphocyte reaction, and d) only Mac-120+ cells display HLA-DS. These studies demonstrate that peripheral adherent mononuclear cells exhibit heterogeneity with regard to their display of Ia antigens. Furthermore, they provide functional data to support the existence of a human Ia determinant, HLA-DS, which is distinct from HLA-DR and which is important in antigen presentation and stimulation in the autologous mixed lymphocyte reaction. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/6184401/Antigen_presenting_capabilities_of_human_monocytes_correlates_with_their_expression_of_HLA_DS_an_Ia_determinant_distinct_from_HLA_DR_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=6184401 DB - PRIME DP - Unbound Medicine ER -