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Inhibition by calcium antagonists of histamine release and calcium influx of rat mast cells: difference between induction of histamine release by concanavalin A and compound 48/80.
Eur J Pharmacol. 1982 Nov 19; 85(2):155-61.EJ

Abstract

The relation between calcium influx and histamine release from rat mast cells was investigated. When purified mast cells pretreated with a calcium antagonist (MnCl2 or methoxyverapamil (D-600)) were exposed to concanavalin A or compound 48/80 in Tyrode solution (pH 7.4) at 37 degrees C, the calcium antagonists inhibited the extracellular calcium-dependent component of concanavalin A-induced histamine release. MnCl2 also inhibited the extracellular calcium-dependent component of compound 48/80-induced histamine release, whereas D-600 did not inhibit the release. D-600 inhibited the 45Ca uptake induced by concanavalin A, but did not inhibit the 45Ca uptake induced by compound 48/80. It was found that the inhibitory action of calcium antagonists depended on the uptake of extracellular calcium. These observations suggest that concanavalin A and compound 48/80 stimulate different mechanisms of calcium influx. Studies on inactivation of the mechanisms of calcium influx showed that calcium influx into cells activated by concanavalin A stopped when concanavalin A was washed out, whereas the influx activated by compound 48/80 was still operative after compound 48/80 had been washed out.

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Publisher Full Text

Authors

Suzuki T
No affiliation info available
Mori K
No affiliation info available
Uchida M
No affiliation info available

MeSH

AnimalsCalciumCalcium Channel BlockersConcanavalin AGallopamilHistamine ReleaseIn Vitro TechniquesMaleManganeseMast CellsRatsp-Methoxy-N-methylphenethylamine

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

6185351

Citation

Suzuki, T, et al. "Inhibition By Calcium Antagonists of Histamine Release and Calcium Influx of Rat Mast Cells: Difference Between Induction of Histamine Release By Concanavalin a and Compound 48/80." European Journal of Pharmacology, vol. 85, no. 2, 1982, pp. 155-61.
Suzuki T, Mori K, Uchida M. Inhibition by calcium antagonists of histamine release and calcium influx of rat mast cells: difference between induction of histamine release by concanavalin A and compound 48/80. Eur J Pharmacol. 1982;85(2):155-61.
Suzuki, T., Mori, K., & Uchida, M. (1982). Inhibition by calcium antagonists of histamine release and calcium influx of rat mast cells: difference between induction of histamine release by concanavalin A and compound 48/80. European Journal of Pharmacology, 85(2), 155-61.
Suzuki T, Mori K, Uchida M. Inhibition By Calcium Antagonists of Histamine Release and Calcium Influx of Rat Mast Cells: Difference Between Induction of Histamine Release By Concanavalin a and Compound 48/80. Eur J Pharmacol. 1982 Nov 19;85(2):155-61. PubMed PMID: 6185351.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition by calcium antagonists of histamine release and calcium influx of rat mast cells: difference between induction of histamine release by concanavalin A and compound 48/80. AU - Suzuki,T, AU - Mori,K, AU - Uchida,M, PY - 1982/11/19/pubmed PY - 1982/11/19/medline PY - 1982/11/19/entrez SP - 155 EP - 61 JF - European journal of pharmacology JO - Eur J Pharmacol VL - 85 IS - 2 N2 - The relation between calcium influx and histamine release from rat mast cells was investigated. When purified mast cells pretreated with a calcium antagonist (MnCl2 or methoxyverapamil (D-600)) were exposed to concanavalin A or compound 48/80 in Tyrode solution (pH 7.4) at 37 degrees C, the calcium antagonists inhibited the extracellular calcium-dependent component of concanavalin A-induced histamine release. MnCl2 also inhibited the extracellular calcium-dependent component of compound 48/80-induced histamine release, whereas D-600 did not inhibit the release. D-600 inhibited the 45Ca uptake induced by concanavalin A, but did not inhibit the 45Ca uptake induced by compound 48/80. It was found that the inhibitory action of calcium antagonists depended on the uptake of extracellular calcium. These observations suggest that concanavalin A and compound 48/80 stimulate different mechanisms of calcium influx. Studies on inactivation of the mechanisms of calcium influx showed that calcium influx into cells activated by concanavalin A stopped when concanavalin A was washed out, whereas the influx activated by compound 48/80 was still operative after compound 48/80 had been washed out. SN - 0014-2999 UR - https://www.unboundmedicine.com/medline/citation/6185351/Inhibition_by_calcium_antagonists_of_histamine_release_and_calcium_influx_of_rat_mast_cells:_difference_between_induction_of_histamine_release_by_concanavalin_A_and_compound_48/80_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0014-2999(82)90460-5 DB - PRIME DP - Unbound Medicine ER -