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Morphogenesis of bacteriophage phi 29 of Bacillus subtilis: oriented and quantized in vitro packaging of DNA protein gp3.
J Virol 1983; 45(1):383-96JV

Abstract

The assembly of phage phi 29 occurs by a single pathway, and the DNA protein (DNA-gp3) of "packaging intermediates" can be obtained after DNase I interruption of in vitro complementation. A broad spectrum of DNA molecules of variable length was isolated from DNase I-treated proheads. Restriction endonuclease EcoRI digestion and electrophoretic analysis of these DNA molecules suggested that DNA-gp3 packaging was oriented with respect to the physical map and was a complex process. Proteinase K-treated exogenous DNA was not packaged. When exogenous DNA-gp3 was predigested with the restriction endonucleases BstEII. EcoRI, HpaI, and HpaII, the left-end fragments, ranging in size from 8 to 0.9 megadaltons, were selectively and efficiently packaged. During in vivo and in vitro assembly, DNA-gp3 is packaged into proheads, the "core-scaffolding" protein gp7 exits from the particles, and the DNA-filled heads assume the angular morphology of phage phi 29. The packaging of a 4.1-megadalton DNA-gp3 left-end fragment (one third of the genome) resulted in the exit of gp7 and the transition to angularity.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

6185695

Citation

Bjornsti, M A., et al. "Morphogenesis of Bacteriophage Phi 29 of Bacillus Subtilis: Oriented and Quantized in Vitro Packaging of DNA Protein Gp3." Journal of Virology, vol. 45, no. 1, 1983, pp. 383-96.
Bjornsti MA, Reilly BE, Anderson DL. Morphogenesis of bacteriophage phi 29 of Bacillus subtilis: oriented and quantized in vitro packaging of DNA protein gp3. J Virol. 1983;45(1):383-96.
Bjornsti, M. A., Reilly, B. E., & Anderson, D. L. (1983). Morphogenesis of bacteriophage phi 29 of Bacillus subtilis: oriented and quantized in vitro packaging of DNA protein gp3. Journal of Virology, 45(1), pp. 383-96.
Bjornsti MA, Reilly BE, Anderson DL. Morphogenesis of Bacteriophage Phi 29 of Bacillus Subtilis: Oriented and Quantized in Vitro Packaging of DNA Protein Gp3. J Virol. 1983;45(1):383-96. PubMed PMID: 6185695.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Morphogenesis of bacteriophage phi 29 of Bacillus subtilis: oriented and quantized in vitro packaging of DNA protein gp3. AU - Bjornsti,M A, AU - Reilly,B E, AU - Anderson,D L, PY - 1983/1/1/pubmed PY - 1983/1/1/medline PY - 1983/1/1/entrez SP - 383 EP - 96 JF - Journal of virology JO - J. Virol. VL - 45 IS - 1 N2 - The assembly of phage phi 29 occurs by a single pathway, and the DNA protein (DNA-gp3) of "packaging intermediates" can be obtained after DNase I interruption of in vitro complementation. A broad spectrum of DNA molecules of variable length was isolated from DNase I-treated proheads. Restriction endonuclease EcoRI digestion and electrophoretic analysis of these DNA molecules suggested that DNA-gp3 packaging was oriented with respect to the physical map and was a complex process. Proteinase K-treated exogenous DNA was not packaged. When exogenous DNA-gp3 was predigested with the restriction endonucleases BstEII. EcoRI, HpaI, and HpaII, the left-end fragments, ranging in size from 8 to 0.9 megadaltons, were selectively and efficiently packaged. During in vivo and in vitro assembly, DNA-gp3 is packaged into proheads, the "core-scaffolding" protein gp7 exits from the particles, and the DNA-filled heads assume the angular morphology of phage phi 29. The packaging of a 4.1-megadalton DNA-gp3 left-end fragment (one third of the genome) resulted in the exit of gp7 and the transition to angularity. SN - 0022-538X UR - https://www.unboundmedicine.com/medline/citation/6185695/Morphogenesis_of_bacteriophage_phi_29_of_Bacillus_subtilis:_oriented_and_quantized_in_vitro_packaging_of_DNA_protein_gp3_ L2 - http://jvi.asm.org/cgi/pmidlookup?view=long&pmid=6185695 DB - PRIME DP - Unbound Medicine ER -