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Splicing of messenger RNA precursors is inhibited by antisera to small nuclear ribonucleoprotein.
Cell. 1983 Nov; 35(1):101-7.Cell

Abstract

A mouse monoclonal antibody and human autoimmune sera directed against various classes of small ribonucleoprotein particles have been tested for inhibition of mRNA splicing in a soluble in vitro system. The splicing of the first and second leader exons of adenovirus late RNA was inhibited only by those sera that reacted with U1 RNP. Both U1 RNP-specific human autoimmune serum and sera directed against the Sm class of small nuclear RNPs, including a mouse monoclonal antibody, specifically inhibited splicing. Antisera specific for U2 RNP had no effect on splicing nor did antisera specific for the La or Ro class of small RNPs. These results suggest that U1 RNP is essential for the splicing of mRNA precursors.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

6194895

Citation

Padgett, R A., et al. "Splicing of Messenger RNA Precursors Is Inhibited By Antisera to Small Nuclear Ribonucleoprotein." Cell, vol. 35, no. 1, 1983, pp. 101-7.
Padgett RA, Mount SM, Steitz JA, et al. Splicing of messenger RNA precursors is inhibited by antisera to small nuclear ribonucleoprotein. Cell. 1983;35(1):101-7.
Padgett, R. A., Mount, S. M., Steitz, J. A., & Sharp, P. A. (1983). Splicing of messenger RNA precursors is inhibited by antisera to small nuclear ribonucleoprotein. Cell, 35(1), 101-7.
Padgett RA, et al. Splicing of Messenger RNA Precursors Is Inhibited By Antisera to Small Nuclear Ribonucleoprotein. Cell. 1983;35(1):101-7. PubMed PMID: 6194895.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Splicing of messenger RNA precursors is inhibited by antisera to small nuclear ribonucleoprotein. AU - Padgett,R A, AU - Mount,S M, AU - Steitz,J A, AU - Sharp,P A, PY - 1983/11/1/pubmed PY - 1983/11/1/medline PY - 1983/11/1/entrez SP - 101 EP - 7 JF - Cell JO - Cell VL - 35 IS - 1 N2 - A mouse monoclonal antibody and human autoimmune sera directed against various classes of small ribonucleoprotein particles have been tested for inhibition of mRNA splicing in a soluble in vitro system. The splicing of the first and second leader exons of adenovirus late RNA was inhibited only by those sera that reacted with U1 RNP. Both U1 RNP-specific human autoimmune serum and sera directed against the Sm class of small nuclear RNPs, including a mouse monoclonal antibody, specifically inhibited splicing. Antisera specific for U2 RNP had no effect on splicing nor did antisera specific for the La or Ro class of small RNPs. These results suggest that U1 RNP is essential for the splicing of mRNA precursors. SN - 0092-8674 UR - https://www.unboundmedicine.com/medline/citation/6194895/Splicing_of_messenger_RNA_precursors_is_inhibited_by_antisera_to_small_nuclear_ribonucleoprotein_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0092-8674(83)90212-X DB - PRIME DP - Unbound Medicine ER -