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Splicing of in vitro synthesized messenger RNA precursors in HeLa cell extracts.
Cell. 1983 Nov; 35(1):89-99.Cell

Abstract

Using a whole cell extract of HeLa cells, we synthesized unspliced RNAs containing the first two leaders and the first intervening sequence of the adenovirus 2 major late transcription unit. Upon incubation of these pre-mRNAs in reaction mixtures containing a nuclear extract and a postnuclear fraction (S100), removal of the first intervening sequence and concomitant joining of the first leader to the second leader was observed. This splicing reaction requires proteins, Mg2+ ions, and ATP. The S100 fraction alone has no splicing activity but stimulates splicing when added to the nuclear extract. Upon fractionation of the postnuclear S100 by chromatography on ion exchange and gel filtration columns, the stimulatory activity copurifies with small ribonucleoprotein particles.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

6194902

Citation

Hernandez, N, and W Keller. "Splicing of in Vitro Synthesized Messenger RNA Precursors in HeLa Cell Extracts." Cell, vol. 35, no. 1, 1983, pp. 89-99.
Hernandez N, Keller W. Splicing of in vitro synthesized messenger RNA precursors in HeLa cell extracts. Cell. 1983;35(1):89-99.
Hernandez, N., & Keller, W. (1983). Splicing of in vitro synthesized messenger RNA precursors in HeLa cell extracts. Cell, 35(1), 89-99.
Hernandez N, Keller W. Splicing of in Vitro Synthesized Messenger RNA Precursors in HeLa Cell Extracts. Cell. 1983;35(1):89-99. PubMed PMID: 6194902.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Splicing of in vitro synthesized messenger RNA precursors in HeLa cell extracts. AU - Hernandez,N, AU - Keller,W, PY - 1983/11/1/pubmed PY - 1983/11/1/medline PY - 1983/11/1/entrez SP - 89 EP - 99 JF - Cell JO - Cell VL - 35 IS - 1 N2 - Using a whole cell extract of HeLa cells, we synthesized unspliced RNAs containing the first two leaders and the first intervening sequence of the adenovirus 2 major late transcription unit. Upon incubation of these pre-mRNAs in reaction mixtures containing a nuclear extract and a postnuclear fraction (S100), removal of the first intervening sequence and concomitant joining of the first leader to the second leader was observed. This splicing reaction requires proteins, Mg2+ ions, and ATP. The S100 fraction alone has no splicing activity but stimulates splicing when added to the nuclear extract. Upon fractionation of the postnuclear S100 by chromatography on ion exchange and gel filtration columns, the stimulatory activity copurifies with small ribonucleoprotein particles. SN - 0092-8674 UR - https://www.unboundmedicine.com/medline/citation/6194902/Splicing_of_in_vitro_synthesized_messenger_RNA_precursors_in_HeLa_cell_extracts_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0092-8674(83)90211-8 DB - PRIME DP - Unbound Medicine ER -