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Ethacrynic acid inhibition of histamine release from rat mast cells: effect on cellular ATP levels and thiol groups.
Eur J Pharmacol. 1983 Sep 02; 92(3-4):181-9.EJ

Abstract

The experiments concerned the effect of ethacrynic acid (0.5 mM) on the adenosine triphosphate (ATP) content of rat mast cells and the effect on histamine release induced by the ionophore A23187 (10 microM). Ethacrynic acid decreased the ATP level of the cells in presence of antimycin A and glucose as well as in presence of 2-deoxyglucose. A23187-induced histamine release was inhibited by ethacrynic acid, and this inhibition was completely reversed by dithiothreitol. These observations may indicate that ethacrynic acid inhibits glycolytic and respiratory energy production in rat mast cells. Furthermore, the inhibition of histamine release occurred mainly through interaction with thiol groups, although inhibition of cellular energy production may have been an additional mechanism.

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Publisher Full Text

Authors

Johansen T
No affiliation info available

MeSH

Adenosine TriphosphateAnimalsCalcimycinCell SurvivalDithiothreitolEthacrynic AcidHistamine ReleaseMaleMast CellsRatsRats, Inbred StrainsSulfhydryl Compounds

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

6195000

Citation

Johansen, T. "Ethacrynic Acid Inhibition of Histamine Release From Rat Mast Cells: Effect On Cellular ATP Levels and Thiol Groups." European Journal of Pharmacology, vol. 92, no. 3-4, 1983, pp. 181-9.
Johansen T. Ethacrynic acid inhibition of histamine release from rat mast cells: effect on cellular ATP levels and thiol groups. Eur J Pharmacol. 1983;92(3-4):181-9.
Johansen, T. (1983). Ethacrynic acid inhibition of histamine release from rat mast cells: effect on cellular ATP levels and thiol groups. European Journal of Pharmacology, 92(3-4), 181-9.
Johansen T. Ethacrynic Acid Inhibition of Histamine Release From Rat Mast Cells: Effect On Cellular ATP Levels and Thiol Groups. Eur J Pharmacol. 1983 Sep 2;92(3-4):181-9. PubMed PMID: 6195000.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ethacrynic acid inhibition of histamine release from rat mast cells: effect on cellular ATP levels and thiol groups. A1 - Johansen,T, PY - 1983/9/2/pubmed PY - 1983/9/2/medline PY - 1983/9/2/entrez SP - 181 EP - 9 JF - European journal of pharmacology JO - Eur J Pharmacol VL - 92 IS - 3-4 N2 - The experiments concerned the effect of ethacrynic acid (0.5 mM) on the adenosine triphosphate (ATP) content of rat mast cells and the effect on histamine release induced by the ionophore A23187 (10 microM). Ethacrynic acid decreased the ATP level of the cells in presence of antimycin A and glucose as well as in presence of 2-deoxyglucose. A23187-induced histamine release was inhibited by ethacrynic acid, and this inhibition was completely reversed by dithiothreitol. These observations may indicate that ethacrynic acid inhibits glycolytic and respiratory energy production in rat mast cells. Furthermore, the inhibition of histamine release occurred mainly through interaction with thiol groups, although inhibition of cellular energy production may have been an additional mechanism. SN - 0014-2999 UR - https://www.unboundmedicine.com/medline/citation/6195000/Ethacrynic_acid_inhibition_of_histamine_release_from_rat_mast_cells:_effect_on_cellular_ATP_levels_and_thiol_groups_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0014-2999(83)90285-6 DB - PRIME DP - Unbound Medicine ER -