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The effect of calmodulin on histamine release in the rat peritoneal mast cell.
Biochim Biophys Acta. 1983 Dec 19; 763(4):419-25.BB

Abstract

To investigate the role of the Ca2+-binding protein calmodulin on histamine release in the rat peritoneal mast cell, we exposed cells to exogenous calmodulin in the presence of a variety of histamine secretagogues. Histamine release stimulated by compound 48/80, polymyxin B and ionophore A23187 was inhibited while concanavalin A-stimulated release was not affected. Calmodulin in the presence of the secretagogues did not affect cell viability and calmodulin alone had no effect on histamine release. No direct interaction between calmodulin and the secretagogues was observed. Exogenous calmodulin does not appear to be incorporated into the cell. The inhibition of histamine release by calmodulin can be explained as a labile interaction between the protein and the cell that requires externally-bound Ca2+. These experiments demonstrate the use of exogenous calmodulin as a probe in the study of the mechanism of histamine release.

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Publisher Full Text

Authors

McClain DE
No affiliation info available
Donlon MA
No affiliation info available
Chock S
No affiliation info available
Catravas GN
No affiliation info available

MeSH

AnimalsCalcimycinCalciumCalmodulinConcanavalin AEgtazic AcidHistamine ReleaseKineticsMaleMast CellsPolymyxin BRatsRats, Inbred Strainsp-Methoxy-N-methylphenethylamine

Pub Type(s)

Journal Article

Language

eng

PubMed ID

6197097

Citation

McClain, D E., et al. "The Effect of Calmodulin On Histamine Release in the Rat Peritoneal Mast Cell." Biochimica Et Biophysica Acta, vol. 763, no. 4, 1983, pp. 419-25.
McClain DE, Donlon MA, Chock S, et al. The effect of calmodulin on histamine release in the rat peritoneal mast cell. Biochim Biophys Acta. 1983;763(4):419-25.
McClain, D. E., Donlon, M. A., Chock, S., & Catravas, G. N. (1983). The effect of calmodulin on histamine release in the rat peritoneal mast cell. Biochimica Et Biophysica Acta, 763(4), 419-25.
McClain DE, et al. The Effect of Calmodulin On Histamine Release in the Rat Peritoneal Mast Cell. Biochim Biophys Acta. 1983 Dec 19;763(4):419-25. PubMed PMID: 6197097.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effect of calmodulin on histamine release in the rat peritoneal mast cell. AU - McClain,D E, AU - Donlon,M A, AU - Chock,S, AU - Catravas,G N, PY - 1983/12/19/pubmed PY - 1983/12/19/medline PY - 1983/12/19/entrez SP - 419 EP - 25 JF - Biochimica et biophysica acta JO - Biochim Biophys Acta VL - 763 IS - 4 N2 - To investigate the role of the Ca2+-binding protein calmodulin on histamine release in the rat peritoneal mast cell, we exposed cells to exogenous calmodulin in the presence of a variety of histamine secretagogues. Histamine release stimulated by compound 48/80, polymyxin B and ionophore A23187 was inhibited while concanavalin A-stimulated release was not affected. Calmodulin in the presence of the secretagogues did not affect cell viability and calmodulin alone had no effect on histamine release. No direct interaction between calmodulin and the secretagogues was observed. Exogenous calmodulin does not appear to be incorporated into the cell. The inhibition of histamine release by calmodulin can be explained as a labile interaction between the protein and the cell that requires externally-bound Ca2+. These experiments demonstrate the use of exogenous calmodulin as a probe in the study of the mechanism of histamine release. SN - 0006-3002 UR - https://www.unboundmedicine.com/medline/citation/6197097/The_effect_of_calmodulin_on_histamine_release_in_the_rat_peritoneal_mast_cell_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0167-4889(83)90104-0 DB - PRIME DP - Unbound Medicine ER -