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Effects of point mutations on formation and structure of the RNA primer for ColE1 DNA replication.
Cell. 1984 Feb; 36(2):513-22.Cell

Abstract

We report mutations affecting several steps of CoIE1 primer formation. Primer precursor forms the hybrid with the template DNA and is cleaved by RNAase H to form the primer. Point mutations at positions -264, -265, -268, and -308 (base pairs upstream of the replication origin) reduce efficiency of hybrid formation. A suppressor mutation at -18 increases the efficiency for a mutant transcript and reduces that for a wild-type transcript. Therefore, hybrid formation probably starts after transcription passes this position. A mutation at -10 affects the site of cleavage by RNAase H. The site appears to be determined by the distance from a stem-loop structure immediately upstream. A double mutation at -186 and -188 results in formation of an RNA secondary structure that prevents use of an RNAase H-cleaved transcript as primer.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

6198094

Citation

Masukata, H, and J Tomizawa. "Effects of Point Mutations On Formation and Structure of the RNA Primer for ColE1 DNA Replication." Cell, vol. 36, no. 2, 1984, pp. 513-22.
Masukata H, Tomizawa J. Effects of point mutations on formation and structure of the RNA primer for ColE1 DNA replication. Cell. 1984;36(2):513-22.
Masukata, H., & Tomizawa, J. (1984). Effects of point mutations on formation and structure of the RNA primer for ColE1 DNA replication. Cell, 36(2), 513-22.
Masukata H, Tomizawa J. Effects of Point Mutations On Formation and Structure of the RNA Primer for ColE1 DNA Replication. Cell. 1984;36(2):513-22. PubMed PMID: 6198094.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of point mutations on formation and structure of the RNA primer for ColE1 DNA replication. AU - Masukata,H, AU - Tomizawa,J, PY - 1984/2/1/pubmed PY - 1984/2/1/medline PY - 1984/2/1/entrez SP - 513 EP - 22 JF - Cell JO - Cell VL - 36 IS - 2 N2 - We report mutations affecting several steps of CoIE1 primer formation. Primer precursor forms the hybrid with the template DNA and is cleaved by RNAase H to form the primer. Point mutations at positions -264, -265, -268, and -308 (base pairs upstream of the replication origin) reduce efficiency of hybrid formation. A suppressor mutation at -18 increases the efficiency for a mutant transcript and reduces that for a wild-type transcript. Therefore, hybrid formation probably starts after transcription passes this position. A mutation at -10 affects the site of cleavage by RNAase H. The site appears to be determined by the distance from a stem-loop structure immediately upstream. A double mutation at -186 and -188 results in formation of an RNA secondary structure that prevents use of an RNAase H-cleaved transcript as primer. SN - 0092-8674 UR - https://www.unboundmedicine.com/medline/citation/6198094/Effects_of_point_mutations_on_formation_and_structure_of_the_RNA_primer_for_ColE1_DNA_replication_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0092-8674(84)90244-7 DB - PRIME DP - Unbound Medicine ER -