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Recipient contributions to serial passive transfer of experimental allergic encephalomyelitis.
J Immunol. 1984 May; 132(5):2417-23.JI

Abstract

EAE can be adoptively transferred into normal recipients by the transfer of BP-specific EAE effector cells. After cell transfer, a series of ill-defined events occur in the recipient that culminate in the development of paralysis associated with neural tissue damage. We investigated the subsequent recipient response to the adoptively transferred disease and examined the role that recipient lymphocytes play in the development of adoptively transferred EAE. Recipient involvement was assessed by the transfer of EAE through a series of normal F1 animals as recipients and at the endpoint of the experiment, determining the MHC restriction pattern of the BP-sensitive cells present. The serial transfer of EAE from BP-CFA-sensitized LEW----(LEW X F-344)F1----(LEW X P2)F1, and from BP-CFA sensitized LEW----(LEW X F344)F1----(LEW X F-344)F1, resulted in the development of BP-sensitive cells in the spleens of the secondary recipients that were able to transfer disease into normal LEW recipients. To test directly for the development of host-derived BP-sensitive cells that might arise in the F1 animal, the serial transfer of EAE from LEW----(LEW X ACI)F1----(LEW X ACI)F1 was performed. When BP-sensitive cells obtained from the secondary (LEW X ACI)F1 recipient animal were transferred into either normal LEW and ACI, or irradiated LEW and ACI animals as final recipients, transfer of disease was successful only into the LEW parental. These results suggest that the development of passive EAE is due solely to the transferred BP-sensitive cells originating from the actively immunized donor, and that no host-derived lymphocytes are recruited into the pool of EAE effector precursor cells found in the spleen of animals after the development of adoptively transferred EAE.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

6201543

Citation

Wegmann, K W., and D J. Hinrichs. "Recipient Contributions to Serial Passive Transfer of Experimental Allergic Encephalomyelitis." Journal of Immunology (Baltimore, Md. : 1950), vol. 132, no. 5, 1984, pp. 2417-23.
Wegmann KW, Hinrichs DJ. Recipient contributions to serial passive transfer of experimental allergic encephalomyelitis. J Immunol. 1984;132(5):2417-23.
Wegmann, K. W., & Hinrichs, D. J. (1984). Recipient contributions to serial passive transfer of experimental allergic encephalomyelitis. Journal of Immunology (Baltimore, Md. : 1950), 132(5), 2417-23.
Wegmann KW, Hinrichs DJ. Recipient Contributions to Serial Passive Transfer of Experimental Allergic Encephalomyelitis. J Immunol. 1984;132(5):2417-23. PubMed PMID: 6201543.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Recipient contributions to serial passive transfer of experimental allergic encephalomyelitis. AU - Wegmann,K W, AU - Hinrichs,D J, PY - 1984/5/1/pubmed PY - 1984/5/1/medline PY - 1984/5/1/entrez SP - 2417 EP - 23 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 132 IS - 5 N2 - EAE can be adoptively transferred into normal recipients by the transfer of BP-specific EAE effector cells. After cell transfer, a series of ill-defined events occur in the recipient that culminate in the development of paralysis associated with neural tissue damage. We investigated the subsequent recipient response to the adoptively transferred disease and examined the role that recipient lymphocytes play in the development of adoptively transferred EAE. Recipient involvement was assessed by the transfer of EAE through a series of normal F1 animals as recipients and at the endpoint of the experiment, determining the MHC restriction pattern of the BP-sensitive cells present. The serial transfer of EAE from BP-CFA-sensitized LEW----(LEW X F-344)F1----(LEW X P2)F1, and from BP-CFA sensitized LEW----(LEW X F344)F1----(LEW X F-344)F1, resulted in the development of BP-sensitive cells in the spleens of the secondary recipients that were able to transfer disease into normal LEW recipients. To test directly for the development of host-derived BP-sensitive cells that might arise in the F1 animal, the serial transfer of EAE from LEW----(LEW X ACI)F1----(LEW X ACI)F1 was performed. When BP-sensitive cells obtained from the secondary (LEW X ACI)F1 recipient animal were transferred into either normal LEW and ACI, or irradiated LEW and ACI animals as final recipients, transfer of disease was successful only into the LEW parental. These results suggest that the development of passive EAE is due solely to the transferred BP-sensitive cells originating from the actively immunized donor, and that no host-derived lymphocytes are recruited into the pool of EAE effector precursor cells found in the spleen of animals after the development of adoptively transferred EAE. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/6201543/Recipient_contributions_to_serial_passive_transfer_of_experimental_allergic_encephalomyelitis_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=6201543 DB - PRIME DP - Unbound Medicine ER -