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[Isolation of mastocytes from rat peritoneal fluid using continuous Ficoll gradients].
Rev Esp Fisiol. 1983 Dec; 39(4):435-40.RE

Abstract

A method for mast cells purification from rat peritoneal fluid is described. The method consists of a continuous Ficoll gradient between 20 and 22,5% (w/v) Ficoll and the cells are obtained with an 88% retrieval. Purity and viability were of 95% and 97% respectively. The cells so purified were functional against the compound 48/80 and the spontaneous secretion value was less than 8%.

Authors

Botana LM
No affiliation info available
Espinosa J
No affiliation info available
Fernández-Otero MP
No affiliation info available

MeSH

AnimalsAscitic FluidCell SeparationCell SurvivalCentrifugation, Density GradientFemaleFicollHistamine ReleaseMaleMast CellsRatsRats, Inbred Strainsp-Methoxy-N-methylphenethylamine

Pub Type(s)

English Abstract
Journal Article

Language

spa

PubMed ID

6201962

Citation

Botana, L M., et al. "[Isolation of Mastocytes From Rat Peritoneal Fluid Using Continuous Ficoll Gradients]." Revista Espanola De Fisiologia, vol. 39, no. 4, 1983, pp. 435-40.
Botana LM, Espinosa J, Fernández-Otero MP. [Isolation of mastocytes from rat peritoneal fluid using continuous Ficoll gradients]. Rev Esp Fisiol. 1983;39(4):435-40.
Botana, L. M., Espinosa, J., & Fernández-Otero, M. P. (1983). [Isolation of mastocytes from rat peritoneal fluid using continuous Ficoll gradients]. Revista Espanola De Fisiologia, 39(4), 435-40.
Botana LM, Espinosa J, Fernández-Otero MP. [Isolation of Mastocytes From Rat Peritoneal Fluid Using Continuous Ficoll Gradients]. Rev Esp Fisiol. 1983;39(4):435-40. PubMed PMID: 6201962.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Isolation of mastocytes from rat peritoneal fluid using continuous Ficoll gradients]. AU - Botana,L M, AU - Espinosa,J, AU - Fernández-Otero,M P, PY - 1983/12/1/pubmed PY - 1983/12/1/medline PY - 1983/12/1/entrez SP - 435 EP - 40 JF - Revista espanola de fisiologia JO - Rev Esp Fisiol VL - 39 IS - 4 N2 - A method for mast cells purification from rat peritoneal fluid is described. The method consists of a continuous Ficoll gradient between 20 and 22,5% (w/v) Ficoll and the cells are obtained with an 88% retrieval. Purity and viability were of 95% and 97% respectively. The cells so purified were functional against the compound 48/80 and the spontaneous secretion value was less than 8%. SN - 0034-9402 UR - https://www.unboundmedicine.com/medline/citation/6201962/[Isolation_of_mastocytes_from_rat_peritoneal_fluid_using_continuous_Ficoll_gradients]_ DB - PRIME DP - Unbound Medicine ER -