TY - JOUR T1 - Mechanism of histamine release from rat isolated peritoneal mast cells by dextran: the role of immunoglobulin E. A1 - Hanahoe,T H, PY - 1984/4/1/pubmed PY - 1984/4/1/medline PY - 1984/4/1/entrez SP - 468 EP - 74 JF - Agents and actions JO - Agents Actions VL - 14 IS - 3-4 N2 - In vivo passive sensitization of rat peritoneal mast cells with Nippostrongylus braziliensis antiserum or rat monoclonal myeloma IgE greatly enhanced histamine release in vitro by dextran or anti IgE, but did not alter release by compound 48/80 or A23187. Conversely, removal of IgE from the cells by acid pH abolished histamine release by dextran and anti IgE but did not impair release by compound 48/80. Whereas, histamine release from cells isolated from rats genetically resistant to dextran (NR rats) by anti IgE was potentiated by passive sensitization, dextran was unable to stimulate secretion from control or sensitized NR cells. The results suggest that dextran releases histamine by interaction with cell-fixed IgE and that the NR mast cell membrane lacks the ability to interpret this stimulus. SN - 0065-4299 UR - https://www.unboundmedicine.com/medline/citation/6203369/Mechanism_of_histamine_release_from_rat_isolated_peritoneal_mast_cells_by_dextran:_the_role_of_immunoglobulin_E_ DB - PRIME DP - Unbound Medicine ER -