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Control of ColE1 plasmid replication: the process of binding of RNA I to the primer transcript.
Cell. 1984 Oct; 38(3):861-70.Cell

Abstract

A transcript (RNA II) from the ColE1 primer promoter forms a persistent hybrid with the template DNA near the replication origin. The hybridized RNA II is cleaved by RNAase H to form the primer. Binding of RNA I to RNA II inhibits the hybridization and thus prevents primer formation. The binding is demonstrated by identification of the product. Analyses of the product with RNAase T1 revealed that pairing initiates at or near the 5' end of RNA I and propagates toward its 3' end. Removal of five nucleotides from the 5' end of RNA I greatly reduces its binding activity. Prior to initiation of pairing, RNA I interacts with RNA II at their single-stranded loops. This interaction is transient but facilitates complete pairing along the entire length of RNA I that initiates from the 5'-end region.

Authors

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Pub Type(s)

Journal Article

Language

eng

PubMed ID

6207934

Citation

Tomizawa, J. "Control of ColE1 Plasmid Replication: the Process of Binding of RNA I to the Primer Transcript." Cell, vol. 38, no. 3, 1984, pp. 861-70.
Tomizawa J. Control of ColE1 plasmid replication: the process of binding of RNA I to the primer transcript. Cell. 1984;38(3):861-70.
Tomizawa, J. (1984). Control of ColE1 plasmid replication: the process of binding of RNA I to the primer transcript. Cell, 38(3), 861-70.
Tomizawa J. Control of ColE1 Plasmid Replication: the Process of Binding of RNA I to the Primer Transcript. Cell. 1984;38(3):861-70. PubMed PMID: 6207934.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Control of ColE1 plasmid replication: the process of binding of RNA I to the primer transcript. A1 - Tomizawa,J, PY - 1984/10/1/pubmed PY - 1984/10/1/medline PY - 1984/10/1/entrez SP - 861 EP - 70 JF - Cell JO - Cell VL - 38 IS - 3 N2 - A transcript (RNA II) from the ColE1 primer promoter forms a persistent hybrid with the template DNA near the replication origin. The hybridized RNA II is cleaved by RNAase H to form the primer. Binding of RNA I to RNA II inhibits the hybridization and thus prevents primer formation. The binding is demonstrated by identification of the product. Analyses of the product with RNAase T1 revealed that pairing initiates at or near the 5' end of RNA I and propagates toward its 3' end. Removal of five nucleotides from the 5' end of RNA I greatly reduces its binding activity. Prior to initiation of pairing, RNA I interacts with RNA II at their single-stranded loops. This interaction is transient but facilitates complete pairing along the entire length of RNA I that initiates from the 5'-end region. SN - 0092-8674 UR - https://www.unboundmedicine.com/medline/citation/6207934/Control_of_ColE1_plasmid_replication:_the_process_of_binding_of_RNA_I_to_the_primer_transcript_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0092-8674(84)90281-2 DB - PRIME DP - Unbound Medicine ER -