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Control of ColE1 plasmid replication: enhancement of binding of RNA I to the primer transcript by the Rom protein.
Cell 1984; 38(3):871-8Cell

Abstract

RNA I prevents a transcript (RNA II) from the ColE1 primer promoter to form a hybrid with the template DNA and thereby inhibits formation of primer for DNA replication. Binding of RNA I to RNA II is responsible for the inhibition. The Rom protein, a plasmid-specified 63 amino acid protein, enhances the inhibitory effect of RNA I on primer formation by enhancing the binding of RNA I to RNA II. In vivo, RNA I controls plasmid copy number and incompatibility and inhibits expression of a galK gene fused to the primer promoter. The rom gene modulates these activities of RNA I. These functions of the rom gene can be explained by alteration of the binding of RNA I to RNA II by the Rom protein.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

6207935

Citation

Tomizawa, J, and T Som. "Control of ColE1 Plasmid Replication: Enhancement of Binding of RNA I to the Primer Transcript By the Rom Protein." Cell, vol. 38, no. 3, 1984, pp. 871-8.
Tomizawa J, Som T. Control of ColE1 plasmid replication: enhancement of binding of RNA I to the primer transcript by the Rom protein. Cell. 1984;38(3):871-8.
Tomizawa, J., & Som, T. (1984). Control of ColE1 plasmid replication: enhancement of binding of RNA I to the primer transcript by the Rom protein. Cell, 38(3), pp. 871-8.
Tomizawa J, Som T. Control of ColE1 Plasmid Replication: Enhancement of Binding of RNA I to the Primer Transcript By the Rom Protein. Cell. 1984;38(3):871-8. PubMed PMID: 6207935.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Control of ColE1 plasmid replication: enhancement of binding of RNA I to the primer transcript by the Rom protein. AU - Tomizawa,J, AU - Som,T, PY - 1984/10/1/pubmed PY - 1984/10/1/medline PY - 1984/10/1/entrez SP - 871 EP - 8 JF - Cell JO - Cell VL - 38 IS - 3 N2 - RNA I prevents a transcript (RNA II) from the ColE1 primer promoter to form a hybrid with the template DNA and thereby inhibits formation of primer for DNA replication. Binding of RNA I to RNA II is responsible for the inhibition. The Rom protein, a plasmid-specified 63 amino acid protein, enhances the inhibitory effect of RNA I on primer formation by enhancing the binding of RNA I to RNA II. In vivo, RNA I controls plasmid copy number and incompatibility and inhibits expression of a galK gene fused to the primer promoter. The rom gene modulates these activities of RNA I. These functions of the rom gene can be explained by alteration of the binding of RNA I to RNA II by the Rom protein. SN - 0092-8674 UR - https://www.unboundmedicine.com/medline/citation/6207935/Control_of_ColE1_plasmid_replication:_enhancement_of_binding_of_RNA_I_to_the_primer_transcript_by_the_Rom_protein_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0092-8674(84)90282-4 DB - PRIME DP - Unbound Medicine ER -