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Morphological aspects of the rat kidney preserved by cold storage. IV. Histoenzymological changes. V. Endopeptidase activity.
Morphol Embryol (Bucur). 1984 Jul-Sep; 30(3):217-21.ME

Abstract

Rat kidneys were preserved by initial washing and cooling perfusion, followed by cold storage at 6 degrees C for 24, 48, 72 and 96 hours, in two different media: Sacks (hyperosmolar electrolytic solution of intracellular type) and Plasmagel (gelation solution 4%). Evidence was found of DPNH-diaphorase, lactate dehydrogenase (LDH), leucylaminopeptidase (LAP) and adenosine-triphosphatase (ATPase) (pH 9.4) activities. Histoenzymological determination showed various levels of enzymatic activities in different segments of the nephron, levels relatively well maintained during storage, even at 72 and 96 hours. At the same time, cathepsin B and D and neutral proteinase activities were determined as parameters of maintained cellular enzymatic activity; different aspects were observed with the two preservation media used.

Authors

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Pub Type(s)

Journal Article

Language

eng

PubMed ID

6239099

Citation

Neagu, S, et al. "Morphological Aspects of the Rat Kidney Preserved By Cold Storage. IV. Histoenzymological Changes. V. Endopeptidase Activity." Morphologie Et Embryologie, vol. 30, no. 3, 1984, pp. 217-21.
Neagu S, Bandu C, Chirculescu AR, et al. Morphological aspects of the rat kidney preserved by cold storage. IV. Histoenzymological changes. V. Endopeptidase activity. Morphol Embryol (Bucur). 1984;30(3):217-21.
Neagu, S., Bandu, C., Chirculescu, A. R., Codorean, E., Cuida, I., & Gabrielescu, E. (1984). Morphological aspects of the rat kidney preserved by cold storage. IV. Histoenzymological changes. V. Endopeptidase activity. Morphologie Et Embryologie, 30(3), 217-21.
Neagu S, et al. Morphological Aspects of the Rat Kidney Preserved By Cold Storage. IV. Histoenzymological Changes. V. Endopeptidase Activity. Morphol Embryol (Bucur). 1984 Jul-Sep;30(3):217-21. PubMed PMID: 6239099.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Morphological aspects of the rat kidney preserved by cold storage. IV. Histoenzymological changes. V. Endopeptidase activity. AU - Neagu,S, AU - Bandu,C, AU - Chirculescu,A R, AU - Codorean,E, AU - Cuida,I, AU - Gabrielescu,E, PY - 1984/7/1/pubmed PY - 1984/7/1/medline PY - 1984/7/1/entrez SP - 217 EP - 21 JF - Morphologie et embryologie JO - Morphol Embryol (Bucur) VL - 30 IS - 3 N2 - Rat kidneys were preserved by initial washing and cooling perfusion, followed by cold storage at 6 degrees C for 24, 48, 72 and 96 hours, in two different media: Sacks (hyperosmolar electrolytic solution of intracellular type) and Plasmagel (gelation solution 4%). Evidence was found of DPNH-diaphorase, lactate dehydrogenase (LDH), leucylaminopeptidase (LAP) and adenosine-triphosphatase (ATPase) (pH 9.4) activities. Histoenzymological determination showed various levels of enzymatic activities in different segments of the nephron, levels relatively well maintained during storage, even at 72 and 96 hours. At the same time, cathepsin B and D and neutral proteinase activities were determined as parameters of maintained cellular enzymatic activity; different aspects were observed with the two preservation media used. SN - 0377-5038 UR - https://www.unboundmedicine.com/medline/citation/6239099/Morphological_aspects_of_the_rat_kidney_preserved_by_cold_storage._IV._Histoenzymological_changes._V._Endopeptidase_activity DB - PRIME DP - Unbound Medicine ER -