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Effects of delta 9-tetrahydrocannabinol and cannabidiol on sodium-dependent high affinity choline uptake in the rat hippocampus.
J Pharmacol Exp Ther 1980; 213(2):216-21JP

Abstract

Doses of delta 9-tetrahydrocannabinol (delta 9-THC) and cannabidiol (CBD) affording the same degree of protection against seizures induced by maximal electroshock were compared for their effects on sodium-dependent high affinity choline uptake into six rat brain regions: cortex, striatum, medulla-pons, hypothalamus, midbrain and hippocampus. One hour after administration of CBD, 60 mg/kg i.p. in vitro choline uptake was not altered in any brain region. In contrast, 1 hr after administration of delta 9-THC, 10 mg/kg i.p., in vitro choline uptake in hippocampus and hypothalamus was significantly reduced. Moreover, in vivo administration of delta 9-THC was followed by a dose-related reduction in in vitro hippocampus choline uptake. Kinetic analysis of hippocampal choline uptake after administration of delta 9-THC, 10 mg/kg i.p., indicated that there was a reduction in Vmax with no change in the Km of the transport system. After direct addition to the hippocampal homogenates (in vitro) both delta 9-THC and CBD inhibited choline uptake, with IC50 values of 4.6 and 15.9 microM, respectively. Kinetic analysis revealed that the in vitro choline uptake inhibition induced by delta 9-THC was noncompetitive in nature. These results suggest that the septal-hippocampal cholinergic tract is a major site of action of delta 9-THC and may provide a neurochemical basis for the differential pharmacological properties of delta 9-THC and CBD.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

6245205

Citation

Lindamood, C, and B K. Colasanti. "Effects of Delta 9-tetrahydrocannabinol and Cannabidiol On Sodium-dependent High Affinity Choline Uptake in the Rat Hippocampus." The Journal of Pharmacology and Experimental Therapeutics, vol. 213, no. 2, 1980, pp. 216-21.
Lindamood C, Colasanti BK. Effects of delta 9-tetrahydrocannabinol and cannabidiol on sodium-dependent high affinity choline uptake in the rat hippocampus. J Pharmacol Exp Ther. 1980;213(2):216-21.
Lindamood, C., & Colasanti, B. K. (1980). Effects of delta 9-tetrahydrocannabinol and cannabidiol on sodium-dependent high affinity choline uptake in the rat hippocampus. The Journal of Pharmacology and Experimental Therapeutics, 213(2), pp. 216-21.
Lindamood C, Colasanti BK. Effects of Delta 9-tetrahydrocannabinol and Cannabidiol On Sodium-dependent High Affinity Choline Uptake in the Rat Hippocampus. J Pharmacol Exp Ther. 1980;213(2):216-21. PubMed PMID: 6245205.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of delta 9-tetrahydrocannabinol and cannabidiol on sodium-dependent high affinity choline uptake in the rat hippocampus. AU - Lindamood,C,3rd AU - Colasanti,B K, PY - 1980/5/1/pubmed PY - 1980/5/1/medline PY - 1980/5/1/entrez SP - 216 EP - 21 JF - The Journal of pharmacology and experimental therapeutics JO - J. Pharmacol. Exp. Ther. VL - 213 IS - 2 N2 - Doses of delta 9-tetrahydrocannabinol (delta 9-THC) and cannabidiol (CBD) affording the same degree of protection against seizures induced by maximal electroshock were compared for their effects on sodium-dependent high affinity choline uptake into six rat brain regions: cortex, striatum, medulla-pons, hypothalamus, midbrain and hippocampus. One hour after administration of CBD, 60 mg/kg i.p. in vitro choline uptake was not altered in any brain region. In contrast, 1 hr after administration of delta 9-THC, 10 mg/kg i.p., in vitro choline uptake in hippocampus and hypothalamus was significantly reduced. Moreover, in vivo administration of delta 9-THC was followed by a dose-related reduction in in vitro hippocampus choline uptake. Kinetic analysis of hippocampal choline uptake after administration of delta 9-THC, 10 mg/kg i.p., indicated that there was a reduction in Vmax with no change in the Km of the transport system. After direct addition to the hippocampal homogenates (in vitro) both delta 9-THC and CBD inhibited choline uptake, with IC50 values of 4.6 and 15.9 microM, respectively. Kinetic analysis revealed that the in vitro choline uptake inhibition induced by delta 9-THC was noncompetitive in nature. These results suggest that the septal-hippocampal cholinergic tract is a major site of action of delta 9-THC and may provide a neurochemical basis for the differential pharmacological properties of delta 9-THC and CBD. SN - 0022-3565 UR - https://www.unboundmedicine.com/medline/citation/6245205/Effects_of_delta_9_tetrahydrocannabinol_and_cannabidiol_on_sodium_dependent_high_affinity_choline_uptake_in_the_rat_hippocampus_ L2 - http://jpet.aspetjournals.org/cgi/pmidlookup?view=long&pmid=6245205 DB - PRIME DP - Unbound Medicine ER -